| RRC ID |
62081
|
| 著者 |
Nakano K, Todo T, Chijiiwa K, Tanaka M.
|
| タイトル |
Therapeutic efficacy of G207, a conditionally replicating herpes simplex virus type 1 mutant, for gallbladder carcinoma in immunocompetent hamsters.
|
| ジャーナル |
Mol Ther
|
| Abstract |
Gallbladder cancer is an extremely difficult disease to cure once metastases occur. In this paper, we explored the potential of G207, an oncolytic, replication-competent herpes simplex virus type 1 mutant, as a new therapeutic means for gallbladder cancer. Gallbladder carcinoma cell lines (four human and one hamster) showed nearly total cell killing within 72 h of G207 infection at a m.o.i. of 0.25 to 2.5 in vitro. The susceptibility to G207 cytopathic activity correlated with the infection efficiency demonstrated by lacZ expression. Intraneoplastic inoculation of G207 (1 x 10(7) pfu) in immunocompetent hamsters bearing established subcutaneous KIGB-5 tumors caused a significant inhibition of tumor growth and prolongation of survival. Repeated inoculations (three times with 4-day intervals) were significantly more efficacious than a single inoculation. In hamsters with bilateral subcutaneous KIGB-5 tumors, inoculation of one tumor alone with G207 caused regression or growth reduction of uninoculated tumors as well as inoculated tumors. In athymic mice, however, the anti-tumor effect was largely reduced in inoculated tumors and completely abolished in remote tumors, suggesting large contribution of T-cell-mediated immune responses to both local and systemic anti-tumor effect of G207. These results indicate that G207 may be useful as a new strategy for gallbladder cancer treatment.
|
| 巻・号 |
3(4)
|
| ページ |
431-7
|
| 公開日 |
2001-4-1
|
| DOI |
10.1006/mthe.2001.0303
|
| PII |
S1525-0016(01)90303-X
|
| PMID |
11319903
|
| MeSH |
Animals
Carcinoma / therapy*
Cell Line
Cricetinae
Female
Gallbladder Neoplasms / therapy*
Genetic Therapy / methods*
Genetic Vectors / genetics
Herpesvirus 1, Human / genetics*
Mesocricetus
Mice
Mice, Nude
Mutation*
Time Factors
Tumor Cells, Cultured
|
| IF |
8.986
|
| リソース情報 |
| ヒト・動物細胞 |
TGBC1TKB(RCB1129)
TGBC2TKB(RCB1130) |
