RRC ID 621
Author Sugimoto M, Okada Y, Sato K, Ito K, Takeda K.
Title A root-specific O-methyltransferase gene expressed in salt-tolerant barley.
Journal Biosci. Biotechnol. Biochem.
Abstract A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves.
Volume 67(5)
Pages 966-72
Published 2003-5
PMID 12834272
MeSH Amino Acid Sequence Blotting, Northern Blotting, Southern Chromosome Mapping DNA, Complementary / biosynthesis DNA, Complementary / genetics DNA, Plant / biosynthesis DNA, Plant / genetics Gene Expression Regulation, Enzymologic / genetics* Gene Expression Regulation, Plant / genetics* Hordeum / enzymology* Hordeum / genetics* Methyltransferases / biosynthesis* Methyltransferases / genetics Molecular Sequence Data Plant Roots / enzymology* RNA, Plant / biosynthesis RNA, Plant / genetics Reverse Transcriptase Polymerase Chain Reaction Sodium Chloride / pharmacology*
IF 1.255
Times Cited 2
WOS Category CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Barley (OU)K305 (OU)I743