| RRC ID |
621
|
| Author |
Sugimoto M, Okada Y, Sato K, Ito K, Takeda K.
|
| Title |
A root-specific O-methyltransferase gene expressed in salt-tolerant barley.
|
| Journal |
Biosci. Biotechnol. Biochem.
|
| Abstract |
A cDNA encoding an O-methyltransferase (OMT) was isolated from salt-tolerant barley roots by subtraction hybridization with cDNAs of salt-tolerant barley roots as a tester cDNA and cDNAs of the salt-sensitive barley roots as a driver cDNA. The deduced amino acid sequence showed significant identity with plant caffeic acid/5-hydroxyferulic acid OMTs. Southern blot analysis showed that the OMT gene was a single copy in both salt-tolerant and -sensitive barley. The cloned gene was expressed in a wheat germ cell-free system to produce the OMT, which had methylating activity for caffeic acid. Northern blot analysis showed that the OMT gene was expressed constitutively in the salt-tolerant barley roots and the expression level was increased 1.5 times by salt stress, but the salt-sensitive barley showed no expression of the gene in roots and leaves.
|
| Volume |
67(5)
|
| Pages |
966-72
|
| Published |
2003-5
|
| PMID |
12834272
|
| MeSH |
Amino Acid Sequence
Blotting, Northern
Blotting, Southern
Chromosome Mapping
DNA, Complementary / biosynthesis
DNA, Complementary / genetics
DNA, Plant / biosynthesis
DNA, Plant / genetics
Gene Expression Regulation, Enzymologic / genetics*
Gene Expression Regulation, Plant / genetics*
Hordeum / enzymology*
Hordeum / genetics*
Methyltransferases / biosynthesis*
Methyltransferases / genetics
Molecular Sequence Data
Plant Roots / enzymology*
RNA, Plant / biosynthesis
RNA, Plant / genetics
Reverse Transcriptase Polymerase Chain Reaction
Sodium Chloride / pharmacology*
|
| IF |
1.297
|
| Times Cited |
2
|
|
WOS Category
|
CHEMISTRY, APPLIED
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Barley |
(OU)K305
(OU)I743 |
