論文 - 詳細
| RRC ID | 62118 |
|---|---|
| 著者 | Yamaguchi T, Yokoyama Y, Ebata T, Matsuda A, Kuno A, Ikehara Y, Shoda J, Narimatsu H, Nagino M. |
| タイトル | Verification of WFA-Sialylated MUC1 as a Sensitive Biliary Biomarker for Human Biliary Tract Cancer. |
| ジャーナル | Ann Surg Oncol |
| Abstract |
BACKGROUND:The diagnostic accuracy of biliary cytology is limited. A novel sandwich enzyme-linked immunosorbent assay that combined Wisteria floribunda agglutinin (WFA) and anti-sialylated mucin 1 (MUC1) monoclonal antibody to target bile samples was recently developed. This study was designed to verify the diagnostic accuracy of WFA-sialylated MUC1 as a sensitive biliary biomarker for human biliary tract cancer. METHODS:Bile samples from 27 patients with benign disease and 174 patients with biliary tract cancer were analyzed. A receiver-operated characteristic curve analysis for biliary WFA-sialylated MUC1 and serum CA19-9 levels was performed to determine the cutoff value for the prediction of the presence of biliary tract cancer. RESULTS:Biliary WFA-sialylated MUC1 levels were significantly higher in the biliary tract cancer group compared with the benign group (P < 0.001). The cutoff value of WFA-sialylated MUC1 for discriminating biliary tract cancer was 10.5. The sensitivity of WFA-sialylated MUC1 in discriminating biliary tract cancer was much higher (82.2 %) than that of cytology (23.6 %) when this cutoff value was used. The cutoff value of serum CA19-9 for discriminating biliary tract cancer was 38 IU/L in the same cohort. All patients with biliary WFA-sialylated MUC1 and serum CA19-9 above the cutoff values had biliary tract cancer, and no patient with benign disease was categorized in this group. CONCLUSIONS:Biliary WFA-sialylated MUC1 is a useful biomarker for the differentiation of biliary tract cancer. The sensitivity of WFA-sialylated MUC1 was clearly higher than that of biliary cytology. Further data collection is necessary to validate the clinical usefulness of this biomarker. |
| 巻・号 | 23(2) |
| ページ | 671-7 |
| 公開日 | 2016-2-1 |
| DOI | 10.1245/s10434-015-4878-4 |
| PII | 10.1245/s10434-015-4878-4 |
| PMID | 26416714 |
| MeSH | Adenocarcinoma, Papillary / blood* Adenocarcinoma, Papillary / secondary Adult Aged Aged, 80 and over Biliary Tract Neoplasms / blood* Biliary Tract Neoplasms / pathology Biomarkers, Tumor / blood* Enzyme-Linked Immunosorbent Assay Female Follow-Up Studies Glycosylation Humans Lymphatic Metastasis Male Middle Aged Mucin-1 / blood* Mucin-1 / chemistry Neoplasm Invasiveness Neoplasm Staging Plant Lectins / chemistry* Prognosis Prospective Studies Receptors, N-Acetylglucosamine / chemistry* |
| IF | 4.061 |
| リソース情報 | |
| ヒト・動物細胞 | TGBC1TKB(RCB1129) |