論文 - 詳細
| RRC ID | 62132 |
|---|---|
| 著者 | Yagi A, Ishizu T, Okamura N, Noguchi S, Itoh H. |
| タイトル | Growth of cultured human bronchiogenic epithelioid CCD-14 Br cells and dermal fibroblasts, NB1 RGB treated with ginseng tetrapeptide and its isomer. |
| ジャーナル | Planta Med |
| Abstract |
The configurations of the component amino acids in ginseng tetrapeptide 1 isolated from Panax ginseng were determined by HPLC with an optical resolution column and the structure of 1 was established to be H-L-Val-gamma-D-Glu-D-Arg- Gly-OH. Synthesis of the ginseng tetrapeptide, 1, and of the configuration and conjugation isomers, H-L-Val-gamma-L-Glu-L-Arg-Gly-OH (2), H-L-Val-D-Glu- D-Arg-Gly-OH (3), and H-L-Val-L-Glu-L-Arg-Gly-OH (4) was carried out by a solid-phase method using the Fmoc strategy. The effects of 1-4 on the proliferation of baby hamster kidney (BHK)-21 cells, normal female bronchiogenic epithelioid (CCD-14 Br) cells, and normal human epidermal fibroblast (NB1 RGB) were examined. Only 1 showed 32 and 23% enhancement of BHK-21 and human CCD-14 Br cells growth, respectively, at a concentration of 13.6 microM and 41% enhancement of NB1 RGB cells growth at a concentration of 32 microM under the conditions employed. It was shown that both the configuration of the component amino acids and the peptide conjugation at a gamma-position of D-Glu in 1 are important for proliferation of the cells. Compound 1 exerted a prominent effect on cell stimulation and growth rate without any morphological change and showed no cytotoxicity. |
| 巻・号 | 62(2) |
| ページ | 115-8 |
| 公開日 | 1996-4-1 |
| DOI | 10.1055/s-2006-957830 |
| PMID | 8657740 |
| MeSH | Amino Acid Sequence Animals Bronchi Cell Division / drug effects* Cell Line Cricetinae Epithelium Female Fibroblasts Humans Isomerism Molecular Sequence Data Oligopeptides / chemical synthesis Oligopeptides / chemistry Oligopeptides / isolation & purification Oligopeptides / pharmacology* Panax* Plants, Medicinal* Skin |
| IF | 2.687 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |