RRC ID 62137
Author Yagi A, Egusa T, Arase M, Tanabe M, Tsuji H.
Title Isolation and characterization of the glycoprotein fraction with a proliferation-promoting activity on human and hamster cells in vitro from Aloe vera gel.
Journal Planta Med
Abstract Fractions of leaf gel from Aloe barbadensis Mill. were prepared by gel permeation using DEAE Sephadex A-25, Sepharose 6B, and Sephadex G-50 columns. These were then tested by in vitro assays for proliferation of human normal dermal and baby hamster kidney cells. The glycoprotein fraction promoted cell growth, while the neutral polysaccharide fraction did not show any growth stimulation. Moreover, the polar-colored glycoprotein fraction strongly inhibited the in vitro assays. An active glycoprotein fraction (protein 82%, carbohydrate 11%) examined on polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE showed a single band. Its molecular weight was 29 kD on a Sephadex G-50 column and its isoelectric point was pH 6.8. Immunoblotting after SDS-PAGE showed that the glycoprotein was composed of two subunits (14 kD). Deglycosylation of glycoprotein (Pg21-2b fraction) by trifluoromethanesulphonic acid provided a protein band with a molecular weight of 13 kD on SDS-PAGE. The colored glycoprotein fraction was shown on SDS-PAGE to be a mixture with a molecular weight of 18 kD-15 kD. It was later hydrolyzed with 10% H2SO4 to produce phenolic substances.
Volume 63(1)
Pages 18-21
Published 1997-2-1
DOI 10.1055/s-2006-957595
PMID 9063091
MeSH Aloe / chemistry* Animals Cell Division / drug effects Cells, Cultured Chromatography, High Pressure Liquid Chromatography, Ion Exchange Cricetinae Electrophoresis, Polyacrylamide Gel Glycoproteins / chemistry Glycoproteins / isolation & purification* Glycoproteins / pharmacology Humans Plants, Medicinal* Skin / cytology Skin / drug effects*
IF 2.687
Human and Animal Cells NB1RGB(RCB0222)