論文 - 詳細
| RRC ID | 62147 |
|---|---|
| 著者 | Yoshimoto K, Tanaka C, Moritani M, Shimizu E, Yamaoka T, Yamada S, Sano T, Itakura M. |
| タイトル | Infrequent detectable somatic mutations of the RET and glial cell line-derived neurotrophic factor (GDNF) genes in human pituitary adenomas. |
| ジャーナル | Endocr J |
| Abstract |
RET is a receptor tyrosine kinase expressed in neuroendocrine cells and tumors. RET is activated by a ligand complex comprising glial cell line-derived neurotrophic factor (GDNF) and GDNF receptor-alpha (GDNFR-alpha). Activating mutations of the RET proto-oncogene were found in multiple endocrine neoplasia (MEN) 2 and in sporadic medullary thyroid carcinoma and pheochromocytoma of neuroendocrine origin. Mutations of the RET proto-oncogene and the glial cell line-derived neurotrophic factor (GDNF) gene were examined in human pituitary tumors. No mutations of the RET proto-oncogene including the cysteine-rich region or codon 768 and 918 in the tyrosine kinase domain were detected in 172 human pituitary adenomas either by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) or by PCR-restriction fragment length polymorphism (RFLP). Further, somatic mutations of the GDNF gene in 33 human pituitary adenomas were not detected by PCR-SSCP. One polymorphism of the GDNF gene at codon 145 of TGC or TGT was observed in a prolactinoma. The RET proto-oncogene message was detected in a normal human pituitary gland or 4 of 4 human pituitary adenomas with reverse transcription (RT)-PCR, and in rodent pituitary tumor cell lines with Western blotting. The expression of GDNF gene was detected in 1 of 4 human somatotroph adenomas, 1 of 2 corticotroph adenomas, and 2 of 6 rodent pituitary tumor cell lines with RT-PCR. Based on these, it is concluded that somatic mutations of the RET proto-oncogene or the GDNF gene do not appear to play a major role in the pituitary tumorigenesis in examined tumors. |
| 巻・号 | 46(1) |
| ページ | 199-207 |
| 公開日 | 1999-2-1 |
| DOI | 10.1507/endocrj.46.199 |
| PMID | 10426588 |
| MeSH | Adenoma / genetics* Animals DNA Mutational Analysis Drosophila Proteins* Gene Expression Glial Cell Line-Derived Neurotrophic Factor Glial Cell Line-Derived Neurotrophic Factor Receptors Humans Mice Mutation* Nerve Growth Factors* Nerve Tissue Proteins / genetics* Pituitary Neoplasms / genetics* Proto-Oncogene Mas Proto-Oncogene Proteins / genetics* Proto-Oncogene Proteins c-ret RNA, Neoplasm / analysis Rats Receptor Protein-Tyrosine Kinases / genetics* Reverse Transcriptase Polymerase Chain Reaction |
| IF | 1.952 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MtT/S(RCB0528) MtT/SM(RCB0530) |