RRC ID 62155
Author Niidome T, Wakamatsu M, Wada A, Hirayama T, Aoyagi H.
Title Required structure of cationic peptide for oligonucleotide-binding and -delivering into cells.
Journal J Pept Sci
Abstract Improvement of the methods for oligonucleotide delivery into cells is necessary for the development of antisense therapy. In the present work, a new strategy for oligonucleotide delivery into cells was tested using cationic peptides as a vector. At first, to understand what structure of the peptide is required for binding with an oligonucleotide, several kinds of alpha-helical and non-alpha-helical peptides containing cationic amino acids were employed. As a result, the amphiphilic alpha-helix peptides were best for binding with the oligonucleotide, and the long chain length and large hydrophobic region in the amphiphilic structure of the peptide were necessary for the binding and forming of aggregates with the oligonucleotide. In the case of non-alpha-helical peptides, no significant binding ability was observed even if their chain lengths and number of cationic amino acid residues were equal to those of the alpha-helical peptides. The remarkable ability of oligonucleotide delivery into COS-7 cells was observed in the alpha-helical peptides with a long chain length and large hydrophobic region in the amphiphilic structure, but was not observed in the non-alpha-helical peptides. It is considered that such alpha-helical peptides could form optimum aggregates with the ODN for uptake into cells. Based on these results, the alpha-helical peptide with a long chain length and large hydrophobic region is applicable as a vector for the delivery of oligonucleotides into cells.
Volume 6(6)
Pages 271-9
Published 2000-6-1
DOI 10.1002/1099-1387(200006)6:6<271::AID-PSC249>3.3.CO;2-6
PMID 10912907
MeSH Amino Acids / chemistry Amino Acids / metabolism Animals COS Cells Cations* Cell Survival / drug effects Circular Dichroism DNA / metabolism Gene Transfer Techniques Genetic Vectors Mass Spectrometry Microscopy, Electron Oligonucleotides / genetics Oligonucleotides / metabolism* Peptides / chemistry* Peptides / metabolism Plasmids / metabolism Protein Binding Transfection
IF 1.877
Human and Animal Cells COS-7(RCB0539)