論文 - 詳細
| RRC ID | 62172 |
|---|---|
| 著者 | Takahashi-Yanaga F, Shiraishi F, Hirata M, Miwa Y, Morimoto S, Sasaguri T. |
| タイトル | Glycogen synthase kinase-3beta is tyrosine-phosphorylated by MEK1 in human skin fibroblasts. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Glycogen synthase kinase-3beta (GSK-3beta) can be associated with several proteins in cell. We analyzed the immunoprecipitates by an anti-GSK-3beta antibody from cell lysate of human fibroblasts and found that this protein was co-precipitated with mitogen-activated protein kinase kinase (MEK1/2). U0126, a MEK1/2 inhibitor, inhibited tyrosine phosphorylation of GSK-3beta, suggesting that MEK1/2 was involved in the phosphorylation of Tyr(216) in GSK-3beta. In vitro kinase assay was carried out using a recombinant human active MEK1 and we found that GSK-3beta was phosphorylated on Tyr(216) by this kinase in a dose- and time-dependent manner. Further, the pretreatment of fibroblasts with U0126 inhibited serum-induced nuclear translocation of GSK-3beta. These results suggested that MEK1/2 induces tyrosine phosphorylation of GSK-3beta and this cellular event might induce nuclear translocation of GSK-3beta. This is the first report to suggest that MEK1/2 phosphorylates not only ERK1/2 but also GSK-3beta. |
| 巻・号 | 316(2) |
| ページ | 411-5 |
| 公開日 | 2004-4-2 |
| DOI | 10.1016/j.bbrc.2004.02.061 |
| PII | S0006291X04003195 |
| PMID | 15020233 |
| MeSH | Active Transport, Cell Nucleus / drug effects Butadienes / pharmacology Cell Nucleus / metabolism Cells, Cultured Enzyme Inhibitors / pharmacology Fibroblasts / drug effects Fibroblasts / enzymology* Glycogen Synthase Kinase 3 / chemistry Glycogen Synthase Kinase 3 / metabolism* Glycogen Synthase Kinase 3 beta Humans MAP Kinase Kinase 1 MAP Kinase Kinase 2 Mitogen-Activated Protein Kinase Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinase Kinases / metabolism* Nitriles / pharmacology Phosphorylation Protein-Tyrosine Kinases / antagonists & inhibitors Protein-Tyrosine Kinases / metabolism Skin / cytology Tyrosine / metabolism* |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |