論文 - 詳細
| RRC ID | 62182 |
|---|---|
| 著者 | Funaba M, Nakaya K, Ikeda T, Murakami M, Tsuchida K, Sugino H. |
| タイトル | Requirement of Smad3 for mast cell growth. |
| ジャーナル | Cell Immunol |
| Abstract |
The involvement of the TGF-beta family in cell growth of bone marrow-derived mast cells (BMMC) cultured with medium containing pokeweed mitogen-stimulated spleen cell-conditioned medium (PWM-SCM) was examined. Doubling time of BMMC from Smad3-null mice was longer than that from wild-type (WT) mice, and the differences tended to be larger with time of culture. Consistent with the results, uptake and reduction of [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] was lower in Smad3-deficient BMMC. Cell cycle analyses revealed no apparent differences between WT BMMC and Smad3-deficient BMMC, suggesting that longer doubling time in Smad3-deficient BMMC resulted from increased cell death. TGF-beta and activin A were supplied by PWM-SCM rather than by self-production by BMMC. Blocking the TGF-beta pathway by anti-TGF-beta neutralizing antibody or an inhibitor for the type I receptors for ligands including TGF-beta and activin, SB431542, inhibited MTS uptake and reduction in WT BMMC, whereas anti-activin A antibody and SB431542 tended to inhibit them in Smad3-deficient BMMC. The present results suggest that TGF-beta-induced and Smad3-mediated signaling is essential for maximal cell growth in mast cells, and that the activin pathway may be required for it when mast cell context is modulated by Smad3 depletion. |
| 巻・号 | 240(1) |
| ページ | 47-52 |
| 公開日 | 2006-3-1 |
| DOI | 10.1016/j.cellimm.2006.06.002 |
| PII | S0008-8749(06)00084-0 |
| PMID | 16839529 |
| MeSH | Activins / metabolism Animals Bone Marrow Cells / cytology Cell Cycle Cell Division Cells, Cultured Culture Media, Conditioned Gene Expression Regulation Inhibin-beta Subunits / metabolism Mast Cells / cytology* Mast Cells / metabolism* Mice Mice, Inbred C57BL Proto-Oncogene Proteins c-kit / genetics RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, IgE / genetics Smad3 Protein / deficiency Smad3 Protein / genetics Smad3 Protein / metabolism* Tetrazolium Salts / metabolism Thiazoles / metabolism Transforming Growth Factor beta / metabolism |
| IF | 4.078 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |