論文 - 詳細
| RRC ID | 62189 |
|---|---|
| 著者 | Hayashi K, Hooper LC, Detrick B, Hooks JJ. |
| タイトル | HSV immune complex (HSV-IgG: IC) and HSV-DNA elicit the production of angiogenic factor VEGF and MMP-9. |
| ジャーナル | Arch Virol |
| Abstract |
Angiogenesis and inflammatory mediators are critical pathogenic factors in herpetic stromal keratitis (HSK). Since disease progresses without infectious virus, HSV-DNA and HSV-IgG complexes (HSV-IC) may contribute to HSK by triggering these factors. Production of VEGF and MMP-9 was studied in vitro using corneal epithelial cells (HCE), fibroblasts (HCRF) and macrophages (THP-1). VEGF was elevated in HCRF and THP-1 following treatment with HSV-DNA and HSV-IC. MMP-9 was elevated in THP-1 but not in corneal cells. When anti-HSV-IgG(Fab')2 complexes stimulated THP-1, MMP-9 was reduced to control levels. Pretreatment of THP-1 with anti-TLR-2 and -3 inhibited MMP-9 production. Thus, HSV-IC may stimulate THP-1 through the Fc receptor and TLRs. Proinflammatory cytokines (IL-1b, IL-6, and TNF-alpha) increased VEGF and MMP-9 in corneal cells and macrophages. These studies indicate that the continued presence of HSV-DNA and HSV-IC contribute to angiogenesis and inflammation in HSK. Thus, cytokines and TLRs may be potential targets for intervention. |
| 巻・号 | 154(2) |
| ページ | 219-26 |
| 公開日 | 2009-1-1 |
| DOI | 10.1007/s00705-008-0303-7 |
| PMID | 19115032 |
| MeSH | Antibodies, Viral / immunology Antigen-Antibody Complex / immunology* Antigen-Antibody Complex / pharmacology Cells, Cultured Cornea / immunology Cornea / virology Corneal Neovascularization / immunology* Corneal Neovascularization / virology Cytokines / pharmacology DNA, Viral / immunology DNA, Viral / pharmacology Fibroblasts / metabolism Fibroblasts / virology Herpesvirus 1, Human / genetics Herpesvirus 1, Human / immunology* Humans Immunoglobulin G / immunology Keratitis, Herpetic / complications Keratitis, Herpetic / immunology* Keratitis, Herpetic / virology Macrophages / immunology Macrophages / virology Matrix Metalloproteinase 9 / biosynthesis* Neutralization Tests Vascular Endothelial Growth Factor A / biosynthesis* |
| IF | 2.243 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |