RRC ID 62232
著者 Ozeki N, Kawai R, Yamaguchi H, Hiyama T, Kinoshita K, Hase N, Nakata K, Kondo A, Mogi M, Nakamura H.
タイトル IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells.
ジャーナル Exp Cell Res
Abstract We reported previously that matrix metalloproteinase (MMP)-13 accelerates bone remodeling in oral periradicular lesions, and indicated a potentially unique role for MMP-13 in wound healing and regeneration of alveolar bone. The ADAM (a disintegrin and metalloprotease) family is a set of multifunctional cell surface and secreted glycoproteins, of which ADAM-28 has been localized in bone and bone-like tissues. In this study, we show that interleukin (IL)-1β induces the expression of MMP-13 and ADAM-28 in homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like (α7(+)hSMSC-OB) cells, and promotes proliferation while inhibiting apoptosis in these cells. At higher concentrations, however, IL-1β failed to induce the expression of these genes and caused an increase in apoptosis. We further employed ADAM-28 small interfering RNA (siRNA) to investigate whether IL-1β-induced MMP-13 expression is linked to this IL-1β-mediated changes in cell proliferation and apoptosis. Silencing ADAM-28 expression potently suppressed IL-1β-induced MMP-13 expression and activity, decreased cell proliferation and increased apoptosis in α7(+)hSMSC-OB cells. In contrast, MMP-13 siRNA had no effect on ADAM-28 expression, suggesting ADAM-28 regulates MMP-13. Exogenous MMP-13 induced α7(+)hSMSC-OB cell proliferation and could rescue ADAM-28 siRNA-induced apoptosis, and we found that proMMP-13 is partially cleaved into its active form by ADAM-28 in vitro. Overall, our results suggest that IL-1β-induced MMP-13 expression and changes in cell proliferation and apoptosis in α7(+)hSMSC-OB cells are regulated by ADAM-28.
巻・号 323(1)
ページ 165-177
公開日 2014-4-15
DOI 10.1016/j.yexcr.2014.02.018
PII S0014-4827(14)00081-0
PMID 24613731
MeSH 3T3 Cells ADAM Proteins / genetics ADAM Proteins / metabolism* Animals Antigens, CD / metabolism Apoptosis / genetics* Cell Line Cell Proliferation DNA Fragmentation Disintegrins / pharmacology Humans Integrin alpha Chains / metabolism Interleukin-1beta / metabolism* Matrix Metalloproteinase 1 / genetics Matrix Metalloproteinase 13 / genetics Matrix Metalloproteinase 13 / metabolism* Membrane Glycoproteins / metabolism Mice Muscle, Skeletal / cytology Osteoblasts / drug effects Osteoblasts / metabolism Platelet Aggregation Inhibitors / metabolism RNA Interference RNA, Small Interfering Stem Cells
IF 3.383
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)