RRC ID |
62232
|
著者 |
Ozeki N, Kawai R, Yamaguchi H, Hiyama T, Kinoshita K, Hase N, Nakata K, Kondo A, Mogi M, Nakamura H.
|
タイトル |
IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells.
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ジャーナル |
Exp Cell Res
|
Abstract |
We reported previously that matrix metalloproteinase (MMP)-13 accelerates bone remodeling in oral periradicular lesions, and indicated a potentially unique role for MMP-13 in wound healing and regeneration of alveolar bone. The ADAM (a disintegrin and metalloprotease) family is a set of multifunctional cell surface and secreted glycoproteins, of which ADAM-28 has been localized in bone and bone-like tissues. In this study, we show that interleukin (IL)-1β induces the expression of MMP-13 and ADAM-28 in homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like (α7(+)hSMSC-OB) cells, and promotes proliferation while inhibiting apoptosis in these cells. At higher concentrations, however, IL-1β failed to induce the expression of these genes and caused an increase in apoptosis. We further employed ADAM-28 small interfering RNA (siRNA) to investigate whether IL-1β-induced MMP-13 expression is linked to this IL-1β-mediated changes in cell proliferation and apoptosis. Silencing ADAM-28 expression potently suppressed IL-1β-induced MMP-13 expression and activity, decreased cell proliferation and increased apoptosis in α7(+)hSMSC-OB cells. In contrast, MMP-13 siRNA had no effect on ADAM-28 expression, suggesting ADAM-28 regulates MMP-13. Exogenous MMP-13 induced α7(+)hSMSC-OB cell proliferation and could rescue ADAM-28 siRNA-induced apoptosis, and we found that proMMP-13 is partially cleaved into its active form by ADAM-28 in vitro. Overall, our results suggest that IL-1β-induced MMP-13 expression and changes in cell proliferation and apoptosis in α7(+)hSMSC-OB cells are regulated by ADAM-28.
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巻・号 |
323(1)
|
ページ |
165-177
|
公開日 |
2014-4-15
|
DOI |
10.1016/j.yexcr.2014.02.018
|
PII |
S0014-4827(14)00081-0
|
PMID |
24613731
|
MeSH |
3T3 Cells
ADAM Proteins / genetics
ADAM Proteins / metabolism*
Animals
Antigens, CD / metabolism
Apoptosis / genetics*
Cell Line
Cell Proliferation
DNA Fragmentation
Disintegrins / pharmacology
Humans
Integrin alpha Chains / metabolism
Interleukin-1beta / metabolism*
Matrix Metalloproteinase 1 / genetics
Matrix Metalloproteinase 13 / genetics
Matrix Metalloproteinase 13 / metabolism*
Membrane Glycoproteins / metabolism
Mice
Muscle, Skeletal / cytology
Osteoblasts / drug effects
Osteoblasts / metabolism
Platelet Aggregation Inhibitors / metabolism
RNA Interference
RNA, Small Interfering
Stem Cells
|
IF |
3.383
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-E1(RCB1126) |