RRC ID |
62237
|
Author |
Ozeki N, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K, Mogi M.
|
Title |
IL-1β-induced, matrix metalloproteinase-3-regulated proliferation of embryonic stem cell-derived odontoblastic cells is mediated by the Wnt5 signaling pathway.
|
Journal |
Exp Cell Res
|
Abstract |
We previously established a method for differentiating induced pluripotent stem cells and embryonic stem (ES) cells into α2 integrin-positive odontoblast-like cells. We also reported that interleukin (IL)-1β induces matrix metalloproteinase (MMP)-3-regulated cell proliferation and suppresses apoptosis in these cells, suggesting that MMP-3 plays a potentially unique physiological role in the regeneration of odontoblast-like cells. Here, we examined whether up-regulation of MMP-3 activity by IL-1β was mediated by Wnt signaling and led to increased proliferation of odontoblast-like cells. IL-1β increased mRNA and protein levels of Wnt5a, Wnt5b and the Wnt receptor Lrp5. Exogenous Wnt5a and Wnt5b were found to increase MMP-3 mRNA, protein and activity, and interestingly the rate of proliferation in these cells. Treatment with siRNAs against Wnt5a, Wnt5b and Lrp5 suppressed the IL-1β-induced increase in MMP-3 expression and suppressed cell proliferation, an effect rescued by application of exogenous Wnt5. These results demonstrate the sequential involvement of Wnt5, Lrp5 and MMP-3 in effecting IL-1β-induced proliferation of ES cell-derived odontoblast-like cells.
|
Volume |
328(1)
|
Pages |
69-86
|
Published |
2014-10-15
|
DOI |
10.1016/j.yexcr.2014.05.006
|
PII |
S0014-4827(14)00197-9
|
PMID |
24851717
|
MeSH |
Animals
Apoptosis
Blotting, Western
Cell Proliferation*
Cells, Cultured
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / metabolism
Low Density Lipoprotein Receptor-Related Protein-5 / antagonists & inhibitors
Low Density Lipoprotein Receptor-Related Protein-5 / genetics
Low Density Lipoprotein Receptor-Related Protein-5 / metabolism
Matrix Metalloproteinase 3 / genetics
Matrix Metalloproteinase 3 / metabolism*
Mice
Odontoblasts / cytology*
Odontoblasts / metabolism
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Wnt Proteins / antagonists & inhibitors
Wnt Proteins / genetics
Wnt Proteins / metabolism*
Wnt-5a Protein
|
IF |
3.383
|
Resource |
Human and Animal Cells |
B6G-2(AES0003) |