RRC ID 62245
著者 Yoshida K, Sato J, Takai R, Uehara O, Kurashige Y, Nishimura M, Chiba I, Saitoh M, Abiko Y.
タイトル Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.
ジャーナル Med Mol Morphol
Abstract Induced pluripotent stem (iPS) cells are generated from adult cells and are potentially of great value in regenerative medicine. Recently, it was shown that iPS cells can differentiate into ameloblast-like cells in cultures using feeder cells. In the present study, we sought to induce differentiation of ameloblast-like cells from iPS cells under feeder-free conditions using medium conditioned by cultured epithelial cell rests of Malassez (ERM) cells and gelatin-coated dishes. Two culture conditions were compared: co-cultures of iPS cells and ERM cells; and, culture of iPS cells in ERM cell-conditioned medium. Differentiation of ameloblast-like cells in the cultures was assessed using real-time RT-PCR assays of expression of the marker genes keratin 14, amelogenin, and ameloblastin and by immunocytochemical staining for amelogenin. We found greater evidence of ameloblast-like cell differentiation in the cultures using the conditioned medium. In the latter, the level of amelogenin expression increased daily and was significantly higher than controls on the 7th, 10th, and 14th days. Expression of ameloblastin also increased daily and was significantly higher than controls on the 14th day. The present study demonstrates that mouse iPS cells can be induced to differentiate into ameloblast-like cells in feeder-free cell cultures using ERM cell-conditioned medium and gelatin-coated dishes.
巻・号 48(3)
ページ 138-45
公開日 2015-9-1
DOI 10.1007/s00795-014-0088-6
PII 10.1007/s00795-014-0088-6
PMID 25319805
MeSH Ameloblasts / metabolism* Amelogenin / genetics Animals Cell Differentiation* Cells, Cultured Culture Media, Conditioned Dental Enamel Proteins / genetics Gene Expression Induced Pluripotent Stem Cells / physiology* Keratin-14 / genetics Mice
IF 2.429
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-178B-5(APS0002)