RRC ID 62256
Author Costa AM, Ferreira RM, Pinto-Ribeiro I, Sougleri IS, Oliveira MJ, Carreto L, Santos MA, Sgouras DN, Carneiro F, Leite M, Figueiredo C.
Title Helicobacter pylori Activates Matrix Metalloproteinase 10 in Gastric Epithelial Cells via EGFR and ERK-mediated Pathways.
Journal J Infect Dis
Abstract Helicobacter pylori colonizes the human stomach and increases the risk for peptic ulcer disease and gastric carcinoma. H. pylori upregulates the expression and activity of several matrix metalloproteinases (MMPs) in cell lines and in the gastric mucosa. The aim of this study was to explore the mechanisms leading to upregulation of MMP10 in gastric epithelial cells induced by H. pylori Infection of gastric cells with H. pylori led to an increase in levels of MMP-10 messenger RNA, protein secretion, and activity. cagA knockout mutants or CagA phosphorylation-defective mutants failed to increase MMP10 expression. These results were confirmed in infection experiments with clinical isolates with known cagA status and in human gastric biopsy specimens. Treatment of cells with chemical inhibitors of the receptor tyrosine kinase EGFR and the kinase Src abrogated H. pylori-induced MMP10 expression. Inhibitors of ERK1/2 and JNK kinases abolished and significantly decreased H. pylori-induced MMP10 expression, respectively, whereas inhibition of the kinase p38 had no effect. Finally, inhibition of MMP10 expression by small interfering RNA led to a decrease in the gastric cell-invasive phenotype mediated by the infection. In conclusion, CagA-positive H. pylori strains stimulate MMP10 expression. MMP-10 modulation occurs via EGFR activation in a process that involves Src, ERK, and JNK pathways. MMP-10 may be implicated in H. pylori-mediated extracellular matrix remodeling.
Volume 213(11)
Pages 1767-76
Published 2016-6-1
DOI 10.1093/infdis/jiw031
PII jiw031
PMID 26802142
MeSH Antigens, Bacterial / metabolism Bacterial Proteins / metabolism Cell Line Cell Line, Tumor Enzyme Activation ErbB Receptors / metabolism* Female Gastric Mucosa / enzymology* Gastric Mucosa / microbiology Helicobacter pylori / pathogenicity* Humans MAP Kinase Kinase 4 / metabolism MAP Kinase Signaling System* Male Matrix Metalloproteinase 10 / metabolism* Middle Aged Virulence Factors / metabolism
IF 5.022
Human and Animal Cells MKN45(RCB1001)