| RRC ID |
62256
|
| 著者 |
Costa AM, Ferreira RM, Pinto-Ribeiro I, Sougleri IS, Oliveira MJ, Carreto L, Santos MA, Sgouras DN, Carneiro F, Leite M, Figueiredo C.
|
| タイトル |
Helicobacter pylori Activates Matrix Metalloproteinase 10 in Gastric Epithelial Cells via EGFR and ERK-mediated Pathways.
|
| ジャーナル |
J Infect Dis
|
| Abstract |
Helicobacter pylori colonizes the human stomach and increases the risk for peptic ulcer disease and gastric carcinoma. H. pylori upregulates the expression and activity of several matrix metalloproteinases (MMPs) in cell lines and in the gastric mucosa. The aim of this study was to explore the mechanisms leading to upregulation of MMP10 in gastric epithelial cells induced by H. pylori Infection of gastric cells with H. pylori led to an increase in levels of MMP-10 messenger RNA, protein secretion, and activity. cagA knockout mutants or CagA phosphorylation-defective mutants failed to increase MMP10 expression. These results were confirmed in infection experiments with clinical isolates with known cagA status and in human gastric biopsy specimens. Treatment of cells with chemical inhibitors of the receptor tyrosine kinase EGFR and the kinase Src abrogated H. pylori-induced MMP10 expression. Inhibitors of ERK1/2 and JNK kinases abolished and significantly decreased H. pylori-induced MMP10 expression, respectively, whereas inhibition of the kinase p38 had no effect. Finally, inhibition of MMP10 expression by small interfering RNA led to a decrease in the gastric cell-invasive phenotype mediated by the infection. In conclusion, CagA-positive H. pylori strains stimulate MMP10 expression. MMP-10 modulation occurs via EGFR activation in a process that involves Src, ERK, and JNK pathways. MMP-10 may be implicated in H. pylori-mediated extracellular matrix remodeling.
|
| 巻・号 |
213(11)
|
| ページ |
1767-76
|
| 公開日 |
2016-6-1
|
| DOI |
10.1093/infdis/jiw031
|
| PII |
jiw031
|
| PMID |
26802142
|
| MeSH |
Antigens, Bacterial / metabolism
Bacterial Proteins / metabolism
Cell Line
Cell Line, Tumor
Enzyme Activation
ErbB Receptors / metabolism*
Female
Gastric Mucosa / enzymology*
Gastric Mucosa / microbiology
Helicobacter pylori / pathogenicity*
Humans
MAP Kinase Kinase 4 / metabolism
MAP Kinase Signaling System*
Male
Matrix Metalloproteinase 10 / metabolism*
Middle Aged
Virulence Factors / metabolism
|
| IF |
5.022
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001) |
