RRC ID 62274
Author Ando Y, Oku T, Tsuji T.
Title Platelet Supernatant Suppresses LPS-Induced Nitric Oxide Production from Macrophages Accompanied by Inhibition of NF-κB Signaling and Increased Arginase-1 Expression.
Journal PLoS One
Abstract We previously reported that mouse bone marrow-derived macrophages (BMDMs) that had been co-cultured with platelets exhibited lower susceptibility to bacterial lipopolysaccharide (LPS) and produced lower levels of nitric oxide (NO) and inflammatory cytokines including TNF-α and IL-6. The suppression of macrophage responses was mediated, at least in part, by platelet supernatant. In the present study, we assessed phenotypic changes of BMDMs induced by incubation with the supernatant from thrombin-activated platelets (PLT-sup) and found that BMDMs cultured with PLT-sup (PLT-BMDMs) expressed a lower level of inducible NO synthase (iNOS) and a higher level of arginase-1, both of which are involved in the L-arginine metabolism, upon stimulation with LPS or zymosan. We also examined possible modulation of the NF-κB signaling pathway and observed suppression of IκBα phosphorylation and a decrease of NF-κB p65 expression in LPS-stimulated PLT-BMDMs. These results suggest that PLT-sup suppresses inflammatory responses of BMDMs via negative regulation of NF-κB signaling leading to lowered expression of iNOS and enhanced L-arginine catabolism by arginase-1.
Volume 11(9)
Pages e0162208
Published 2016-1-1
DOI 10.1371/journal.pone.0162208
PII PONE-D-16-20239
PMID 27588757
PMC PMC5010197
MeSH Animals Arginase / metabolism* Blood Platelets / metabolism* Coculture Techniques Lipopolysaccharides / pharmacology* Macrophages / drug effects* Macrophages / metabolism Mice Mice, Inbred BALB C NF-kappa B / metabolism* Nitric Oxide / metabolism* Signal Transduction / drug effects*
IF 2.74
Human and Animal Cells L929(RCB2619)