RRC ID 62332
著者 Nakagawa T, Fujita N, Oh-Hara T, Kurokawa T, Nakamura K, Tsuruo T.
タイトル Interleukin-1 alpha induced cyclooxygenase-2 expression in bone-derived endothelial cells.
ジャーナル J Cell Physiol
Abstract Histological studies have suggested that vascular endothelial cells in bone are members of a complex network that regulates bone development and remodeling by producing soluble factors or by mediating cell-cell adhesion. To clarify the role of bone-derived endothelial cell lines (BDECs) in bone remodeling, we established several clones of BDECs from the femurs of BALB/c mice after transformation with the SV40 virus. Then we examined the response of these clones to interleukin-1alpha (IL-1alpha). IL-1alpha is known to induce bone resorption in part by increasing the expression of cyclooxygenase-2 (COX-2) that is associated with the production of PGE2 in osteoblast-lineage cells. Treating the primary and established BDECs with IL-1alpha induced COX-2 mRNA expression. A transcriptional activation assay revealed that the treatment with IL-1alpha increased COX-2 promoter activity in a dose-dependent manner, and IL-1alpha promoted COX-2 protein expression in BDECs. Treatment with IL-1alpha promoted PGE2 production from BDECs in a dose-dependent manner. These results indicate that IL-1alpha stimulates PGE2 synthesis largely by inducing BDECs to express COX-2. Because PGE2 stimulates bone resorption, these vascular endothelial cells, as well as osteoblast cells, play important roles in bone remodeling.
巻・号 179(2)
ページ 226-32
公開日 1999-5-1
DOI 10.1002/(SICI)1097-4652(199905)179:2<226::AID-JCP13>3.0.CO;2-Q
PII 10.1002/(SICI)1097-4652(199905)179:2<226::AID-JCP13>3.0.CO;2-Q
PMID 10199562
MeSH Animals Bone Remodeling / drug effects* Cell Line Cyclooxygenase 2 Dinoprostone / metabolism Endothelium, Vascular / drug effects* Femur / metabolism Gene Expression Regulation, Enzymologic / drug effects Interleukin-1 / pharmacology* Isoenzymes / biosynthesis* Mice Mice, Inbred BALB C Prostaglandin-Endoperoxide Synthases / biosynthesis* RNA, Messenger / metabolism Receptors, Interleukin-1 / drug effects Transfection Transformation, Genetic
IF 5.546
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)