| RRC ID |
6236
|
| Author |
Shi X, Teo LS, Pan X, Chong SW, Kraut R, Korzh V, Wohland T.
|
| Title |
Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy.
|
| Journal |
Dev Dyn
|
| Abstract |
Zebrafish and Drosophila are animal models widely used in developmental biology. High-resolution microscopy and live imaging techniques have allowed the investigation of biological processes down to the cellular level in these models. Here, using fluorescence correlation spectroscopy (FCS), we show that even processes on a molecular level can be studied in these embryos. The two animal models provide different advantages and challenges. We first characterize their autofluorescence pattern and determine usable penetration depth for FCS especially in the case of zebrafish, where tissue thickness is an issue. Next, the applicability of FCS to study molecular processes is shown by the determination of blood flow velocities with high spatial resolution and the determination of diffusion coefficients of cytosolic and membrane-bound enhanced green fluorescent protein-labeled proteins in different cell types. This work provides an approach to study molecular processes in vivo and opens up the possibility to relate these molecular processes to developmental biology questions.
|
| Volume |
238(12)
|
| Pages |
3156-67
|
| Published |
2009-12-1
|
| DOI |
10.1002/dvdy.22140
|
| PMID |
19882725
|
| MeSH |
Animals
Animals, Genetically Modified
Diffusion
Drosophila melanogaster / embryology*
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism
Embryo, Nonmammalian / blood supply
Embryonic Development / physiology*
Female
Fluorescence
Fluorescent Antibody Technique / instrumentation
Fluorescent Antibody Technique / methods
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Male
Models, Biological
Regional Blood Flow / physiology
Sensitivity and Specificity
Spectrometry, Fluorescence / methods
Zebrafish / embryology*
Zebrafish / genetics
Zebrafish / metabolism
|
| IF |
3.275
|
| Times Cited |
25
|
|
WOS Category
|
ANATOMY & MORPHOLOGY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Zebrafish |
rw0(Tg(CM-isl1:GFP)) |
