RRC ID 62517
Author Watanabe T, Yamazaki S, Yoneda N, Shinohara H, Tomioka I, Higuchi Y, Yagoto M, Ema M, Suemizu H, Kawai K, Sasaki E.
Title Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds.
Journal Genes Cells
Abstract Induced pluripotent stem (iPS) cells hold great promise for regenerative medicine and the treatment of various diseases. Before proceeding to clinical trials, it is important to test the efficacy and safety of iPS cell-based treatments using experimental animals. The common marmoset is a new world monkey widely used in biomedical studies. However, efficient methods that could generate iPS cells from a variety of cells have not been established. Here, we report that marmoset cells are efficiently reprogrammed into iPS cells by combining RNA transfection and chemical compounds. Using this novel combination, we generate transgene integration-free marmoset iPS cells from a variety of cells that are difficult to reprogram using conventional RNA transfection method. Furthermore, we show this is similarly effective for human and cynomolgus monkey iPS cell generation. Thus, the addition of chemical compounds during RNA transfection greatly facilitates reprogramming and efficient generation of completely integration-free safe iPS cells in primates, particularly from difficult-to-reprogram cells.
Volume 24(7)
Pages 473-484
Published 2019-7-1
DOI 10.1111/gtc.12702
PMID 31099158
PMC PMC6852476
MeSH Aged Animals Cell Differentiation Cells, Cultured Cellular Reprogramming* Female Fibroblasts / cytology* Fibroblasts / drug effects Fibroblasts / physiology Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / physiology Pharmaceutical Preparations / administration & dosage* Platyrrhini RNA / administration & dosage* Transfection / methods*
IF 1.922
DNA material phBG (RDB18330) phBG-p53DD (RDB18331)