RRC ID 6254
著者 Kapsimali M, Kloosterman WP, de Bruijn E, Rosa F, Plasterk RH, Wilson SW.
タイトル MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system.
ジャーナル Genome Biol
Abstract BACKGROUND:MicroRNA (miRNA) encoding genes are abundant in vertebrate genomes but very few have been studied in any detail. Bioinformatic tools allow prediction of miRNA targets and this information coupled with knowledge of miRNA expression profiles facilitates formulation of hypotheses of miRNA function. Although the central nervous system (CNS) is a prominent site of miRNA expression, virtually nothing is known about the spatial and temporal expression profiles of miRNAs in the brain. To provide an overview of the breadth of miRNA expression in the CNS, we performed a comprehensive analysis of the neuroanatomical expression profiles of 38 abundant conserved miRNAs in developing and adult zebrafish brain.
RESULTS:Our results show miRNAs have a wide variety of different expression profiles in neural cells, including: expression in neuronal precursors and stem cells (for example, miR-92b); expression associated with transition from proliferation to differentiation (for example, miR-124); constitutive expression in mature neurons (miR-124 again); expression in both proliferative cells and their differentiated progeny (for example, miR-9); regionally restricted expression (for example, miR-222 in telencephalon); and cell-type specific expression (for example, miR-218a in motor neurons).
CONCLUSION:The data we present facilitate prediction of likely modes of miRNA function in the CNS and many miRNA expression profiles are consistent with the mutual exclusion mode of function in which there is spatial or temporal exclusion of miRNAs and their targets. However, some miRNAs, such as those with cell-type specific expression, are more likely to be co-expressed with their targets. Our data provide an important resource for future functional studies of miRNAs in the CNS.
巻・号 8(8)
ページ R173
公開日 2007-1-1
DOI 10.1186/gb-2007-8-8-r173
PII gb-2007-8-8-r173
PMID 17711588
PMC PMC2375003
MeSH Animals Brain / cytology Brain / growth & development* Cell Differentiation / genetics Gene Expression Profiling Gene Expression Regulation, Developmental* Larva / chemistry Larva / cytology Larva / genetics Larva / growth & development MicroRNAs / analysis MicroRNAs / genetics* MicroRNAs / metabolism Oligonucleotide Array Sequence Analysis Organogenesis / genetics* Zebrafish / genetics Zebrafish / growth & development*
IF 10.806
引用数 277
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY
リソース情報
ゼブラフィッシュ rw0 (Tg(CM-isl1:GFP))