RRC ID 62542
Author Joshi R, Thakuri PS, Buchanan JC, Li J, Tavana H.
Title Microprinted Stem Cell Niches Reveal Compounding Effect of Colony Size on Stromal Cells-Mediated Neural Differentiation.
Journal Adv Healthc Mater
Abstract Microenvironmental factors have a major impact on differentiation of embryonic stem cells (ESCs). Here, a novel phenomenon that size of ESC colonies has a significant regulatory role on stromal cells induced differentiation of ESCs to neural cells is reported. Using a robotic cell microprinting technology, defined densities of ESCs are confined within aqueous nanodrops over a layer of supporting stromal cells immersed in a second, immiscible aqueous phase to generate ESC colonies of defined sizes. Temporal protein and gene expression studies demonstrate that larger ESC colonies generate disproportionally more neural cells and longer neurite processes. Unlike previous studies that attribute neural differentiation of ESCs solely to interactions with stromal cells, it is found that increased intercellular signaling of ESCs significantly enhances neural differentiation. This study offers an approach to generate neural cells with improved efficiency for potential use in translational research.
Volume 7(5)
Published 2018-3-1
DOI 10.1002/adhm.201700832
PMID 29193846
PMC PMC5842135
MeSH Animals Cell Communication* Cell Differentiation* Cells, Cultured Mice Mouse Embryonic Stem Cells / cytology Mouse Embryonic Stem Cells / metabolism* Neural Stem Cells / cytology Neural Stem Cells / metabolism* Signal Transduction* Stem Cell Niche Stromal Cells / cytology Stromal Cells / metabolism
IF 7.367
Resource
Human and Animal Cells EB5(AES0151) MC3T3-G2/PA6(RCB1127)