論文 - 詳細
| RRC ID | 62618 |
|---|---|
| 著者 | Hikita T, Miyata M, Watanabe R, Oneyama C. |
| タイトル | In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter. |
| ジャーナル | Sci Rep |
| Abstract |
Monitoring of exosome dynamics in living organisms is essential to demonstrate the real functions of cancer-derived exosomes. Currently, these have been elucidated in vitro or under non-physiological conditions in vivo in most cases. To overcome these limitations, we developed an imaging method using Antares2-mediated bioluminescence resonance energy transfer (BRET) for observing long-term accumulation of exosomes in vivo. Ectopic expression of CD63-Antares2 effectively labeled exosomes with Antares2, which emitted intense, long-wavelength luminescence suitable for in vivo monitoring. Transplantation of CD63-Antares2-expressing prostate cancer cells into mice allowed determining the amount of cancer-derived exosomes released from primary tumors into the bloodstream and visualizing the long-term homing behavior of exosomes to their target organs or tissues. Interestingly, secreted exosome was decreased upon administration of low dose of dasatinib, an approved tyrosine-kinase inhibitor. The CD63-Antares2 xenograft mouse model will be useful for elucidating the dynamics of cancer-derived exosomes in vivo and evaluating the therapeutic efficacy and mechanism of exosome production inhibitors. |
| 巻・号 | 10(1) |
| ページ | 16616 |
| 公開日 | 2020-10-6 |
| DOI | 10.1038/s41598-020-73580-5 |
| PII | 10.1038/s41598-020-73580-5 |
| PMID | 33024173 |
| PMC | PMC7538576 |
| MeSH | Animals Bioluminescence Resonance Energy Transfer Techniques / methods* Dasatinib / pharmacology Energy Transfer* Exosomes / metabolism* Heterografts Male Mice Molecular Imaging / methods* Neoplasm Transplantation Prostatic Neoplasms / metabolism* Prostatic Neoplasms / pathology* Protein Kinase Inhibitors / pharmacology Time Factors |
| IF | 3.998 |
| リソース情報 | |
| 遺伝子材料 | pcDNA3 Venus-Akaluc (RDB15781) |
| ヒト・動物細胞 | DU145(RCB2143) LNCap.FGC(RCB2144) PC-3(RCB2145) |