論文 - 詳細
| RRC ID | 62709 |
|---|---|
| 著者 | Anuchapreeda S, Rungrojsakul M, Tima S, Chiampanichayakul S, Krig SR. |
| タイトル | Co-activation of WT1 and AP-1 proteins on WT1 gene promoter to induce WT1 gene expression in K562 cells. |
| ジャーナル | Cell Signal |
| Abstract |
Earlier studies have revealed one function of the inhibitory mechanism of curcumin. Activating PKCα induces WT1 gene expression via signalling through downstream JNK and c-JUN. In the present study, the effect of c-JUN/AP-1 binding and transcriptional regulation of the WT1 gene promoter was investigated in K562 leukaemic cells. The non-cytotoxic dose (IC20 values) of curcumin (WT1 and AP-1 inhibitors) was employed to examine its effect on WT1 gene-mediated WT1 and AP-1 protein expression. Non-cytotoxic doses of both tanshinone IIA (AP-1 DNA-binding inhibitor) and SP600125 (JNK inhibitor) were used to test the role of c-JUN/AP-1 in WT1 gene expression. Curcumin, tanshinone IIA, and SP600125 inhibited WT1 protein expression in a dose-dependent manner (5-15 μM) at 24 h as shown by immunoblotting. A ChIP assay showed that curcumin and tanshinone IIA inhibited AP-1 and WT1 binding to the proximal WT1 promoter (-301 bp), and a luciferase reporter assay showed that the WT1 luciferase gene reporter activity was decreased after curcumin, tanshinone IIA, and SP600126 treatments. Furthermore, depletion of c-JUN abrogated WT1 gene expression. In summary, AP-1 contributes to the WT1 autoregulation of WT1 gene expression in leukaemic K562 cells. |
| 巻・号 | 53 |
| ページ | 339-347 |
| 公開日 | 2019-1-1 |
| DOI | 10.1016/j.cellsig.2018.11.001 |
| PII | S0898-6568(18)30271-7 |
| PMID | 30395942 |
| MeSH | Abietanes / pharmacology Anthracenes / pharmacology Antineoplastic Agents / pharmacology Curcumin / pharmacology Gene Expression Regulation, Leukemic* / drug effects Humans K562 Cells Leukemia / drug therapy Leukemia / genetics* Promoter Regions, Genetic / drug effects Protein Kinase Inhibitors / pharmacology Transcription Factor AP-1 / genetics* WT1 Proteins / genetics* |
| IF | 3.968 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB1897) |