論文 - 詳細
| RRC ID | 62722 |
|---|---|
| 著者 | Suzuki R, Aruga A, Kobayashi H, Yamato M, Yamamoto M. |
| タイトル | Development of a novel in vivo cancer model using cell sheet engineering. |
| ジャーナル | Anticancer Res |
| Abstract |
AIM:Standard in vivo cancer models entail injecting single cancer cells, but this technique is not always successful. We developed a novel cancer cell sheet by using temperature-responsive polymer poly(N-isopropyl acryl amide)-coated plates, which allow controlled attachment and detachment of living cancer cells via simple temperature changes. MATERIALS AND METHODS:Four human cancer cell lines were used to make cell sheets. The cancer cell sheets were subcutaneously transplanted into nude mice and compared regarding their tumor-forming ability with the conventional cell suspension technique. RESULTS:Human cancer cell sheets were successfully transplanted into nude mice. The cancer cell sheets resulted in stable engraftment and showed a higher tumor volume determined by total flux with the IVIS® imaging system. CONCLUSION:Novel cancer cell sheets are useful tools to make in vivo cancer models in mice for the assessment of anticancer therapeutics. |
| 巻・号 | 34(9) |
| ページ | 4747-54 |
| 公開日 | 2014-9-1 |
| PII | 34/9/4747 |
| PMID | 25202053 |
| MeSH | Animals Cell Culture Techniques* Cell Line, Tumor Cell Proliferation Female HCT116 Cells Humans Mice Neoplasms / pathology Tumor Cells, Cultured Xenograft Model Antitumor Assays |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | Li-7(RCB1941) MKN74(RCB1002) |