RRC ID 6274
著者 Igawa T, Fujiwara M, Takahashi H, Sawasaki T, Endo Y, Seki M, Shinozaki K, Fukao Y, Yanagawa Y.
タイトル Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings.
ジャーナル J Exp Bot
Abstract The majority of proteins in eukaryotic cells are modified according to highly regulated mechanisms to fulfill specific functions and to achieve localization, stability, and transport. Protein ubiquitination is one of the major post-translational modifications occurring in eukaryotic cells. To obtain the proteomic dataset related to the ubiquitin (Ub)-dependent regulatory system in Arabidopsis, affinity purification with an anti-Ub antibody under native condition was performed. Using MS/MS analysis, 196 distinct proteins represented by 251 distinct genes were identified. The identified proteins were involved in metabolism (23.0%), stress response (21.4%), translation (16.8%), transport (6.7%), cell morphology (3.6%), and signal transduction (1.5%), in addition to proteolysis (16.8%) to which proteasome subunits (14.3%) is included. On the basis of potential ubiquitination-targeting signal motifs, in-gel mobilities, and previous reports, 78 of the identified proteins were classified as ubiquitinated proteins and the rest were speculated to be associated proteins of ubiquitinated proteins. The degradation of three proteins predicted to be ubiquitinated proteins was inhibited by a proteasome inhibitor, suggesting that the proteins were regulated by Ub/proteasome-dependent proteolysis.
巻・号 60(11)
ページ 3067-73
公開日 2009-1-1
DOI 10.1093/jxb/erp134
PII erp134
PMID 19429840
PMC PMC2718211
MeSH Arabidopsis / chemistry* Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis Proteins / chemistry* Arabidopsis Proteins / genetics Arabidopsis Proteins / isolation & purification* Arabidopsis Proteins / metabolism Proteomics Seedlings / chemistry Seedlings / genetics Seedlings / metabolism Ubiquitin / metabolism* Ubiquitination
IF 5.908
引用数 45
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda13478