RRC ID 6276
著者 Kang K, Park M, Park S, Kim YS, Lee S, Lee SG, Back K.
タイトル Production of plant-specific tyramine derivatives by dual expression of tyramine N-hydroxycinnamoyltransferase and 4-coumarate:coenzyme A ligase in Escherichia coli.
ジャーナル Biotechnol Lett
Abstract Plant-specific bioactive compounds including feruloyltyramine (FT), 4-coumaroyltyramine (CT), and caffeoyltyramine (CaT) were simultaneously produced in Escherichia coli by heterologous expression of two biosynthetic genes encoding 4-coumarate:coenzyme A ligase and tyramine N-hydroxycinnamoyltransferase (THT) cloned from Arabidopsis thaliana and pepper, respectively. Simultaneous supplementation of substrates to the recombinant E. coli resulted in the secretion of multiple tyramine derivatives into the medium at high yield: CT (189 mg l(-1)), FT (135 mg l(-1)), CaT (40 mg l(-1)). In addition, the recombinant E. coli also produced, albeit at low concentration, a range of dopamine derivatives such as feruloyldopamine due to THT's ability to accept dopamine as a substrate.
巻・号 31(9)
ページ 1469-75
公開日 2009-9-1
DOI 10.1007/s10529-009-0032-3
PMID 19479321
MeSH Acyltransferases / genetics* Acyltransferases / metabolism* Arabidopsis / enzymology* Arabidopsis / genetics Caffeic Acids / metabolism Coenzyme A Ligases / genetics* Coenzyme A Ligases / metabolism* Coumaric Acids / metabolism Culture Media / chemistry Escherichia coli / genetics Escherichia coli / metabolism* Gene Expression Recombinant Proteins / genetics Recombinant Proteins / metabolism Tyramine / analogs & derivatives* Tyramine / metabolism*
IF 1.977
引用数 3
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda08860