論文 - 詳細
| RRC ID | 62770 |
|---|---|
| 著者 | Tang B, Lei B, Qi G, Liang X, Tang F, Yuan S, Wang Z, Yu S, He S. |
| タイトル | MicroRNA-155-3p promotes hepatocellular carcinoma formation by suppressing FBXW7 expression. |
| ジャーナル | J Exp Clin Cancer Res |
| Abstract |
BACKGROUND:MicroRNAs (miRNAs) are small non-coding RNAs frequently dysregulated in human malignant tumors. In the present study, we analyzed the role miR-155-3p plays in Hepatocellular carcinoma (HCC), which has been reported participation in some other types of cancer. METHODS:qRT-PCR was used to measure the levels of miR-155-3p in HCC specimens and HCC cell lines. Overexpression of miR-155-3p and miR-155-3p inhibitor were transfected into HCC cell lines to investigate its role in HCC. Colony formation assay and 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays were used to analyses cell proliferation in vitro. In vivo tumor formation assays were performed in BALB/c nude mice. Luciferase reporter assay was carried out to measure the translation of F-Box and WD repeat romain containing 7 (FBXW7). RESULTS:We found that miR-155-3p was remarkably upregulated both in HCC tissue and cell lines. Overexpression of miR-155-3p enhanced HCC cell proliferation in vitro and tumorigenesis in vivo. In addition, overexpression of miR-155-3p is correlated with decreased levels FBXW7 mainly through inhibiting the expression of FBXW7. CONCLUSIONS:Our studies suggest that miR-155-3p plays an important role in the pathogenesis of HCC and implicates its potential applications in the treatment of HCC cancer. |
| 巻・号 | 35(1) |
| ページ | 93 |
| 公開日 | 2016-6-16 |
| DOI | 10.1186/s13046-016-0371-6 |
| PII | 10.1186/s13046-016-0371-6 |
| PMID | 27306418 |
| PMC | PMC4910248 |
| MeSH | 3' Untranslated Regions Animals Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / pathology* Cell Line, Tumor Cell Proliferation F-Box-WD Repeat-Containing Protein 7 / genetics* Female Gene Expression Regulation, Neoplastic Hep G2 Cells Humans Liver Neoplasms / genetics Liver Neoplasms / pathology* Male Mice Mice, Nude MicroRNAs / genetics* Neoplasm Transplantation Protein Biosynthesis Up-Regulation |
| IF | 7.068 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |