論文 - 詳細
| RRC ID | 62783 |
|---|---|
| 著者 | Kushitani K, Amatya VJ, Mawas AS, Miyata Y, Okada M, Takeshima Y. |
| タイトル | Use of Anti-Noxa Antibody for Differential Diagnosis between Epithelioid Mesothelioma and Reactive Mesothelial Hyperplasia. |
| ジャーナル | Pathobiology |
| Abstract |
OBJECTIVES:The histological differential diagnosis between epithelioid mesothelioma (EM) and reactive mesothelial hyperplasia (RMH) is not always straightforward. The aim of the present study was to search for new immunohistochemical markers to distinguish EM from RMH. METHODS:We evaluated and compared the expression of apoptosis-related genes in EM and RMH by real-time RT-PCR array analysis followed by clustering of significant gene expression. Immunohistochemical staining and statistical analysis of Noxa expression in 81 cases of EM and 55 cases of RMH were performed and compared with the utility of other previously reported antibodies such as Desmin, EMA, GLUT-1, IMP-3 and CD146. RESULTS:Noxa mRNA expression levels were found to be increased in EM when compared to RMH by RT-PCR array analysis. In the immunohistochemical analysis, Noxa showed sensitivity of 69.0%, specificity of 93.6% and positive predictive value of 93.0% as a positive marker of EM in distinguishing it from RMH, and these values were almost similar to IMP-3. CONCLUSION:Noxa is a marker with relatively high specificity, and can be used to distinguish EM from RMH. It would be a valuable addition to the current antibody panel used for the differential diagnosis of EM and RMH. |
| 巻・号 | 83(1) |
| ページ | 33-40 |
| 公開日 | 2016-1-1 |
| DOI | 10.1159/000442092 |
| PII | 000442092 |
| PMID | 26735863 |
| MeSH | Antibodies Apoptosis / genetics* Biomarkers, Tumor / analysis* Cell Line, Tumor Desmin / analysis Desmin / immunology Diagnosis, Differential Female Gene Expression Profiling Glucose Transporter Type 1 / analysis Glucose Transporter Type 1 / immunology Humans Hyperplasia / diagnosis* Immunohistochemistry Lung Neoplasms / diagnosis* Lung Neoplasms / ultrastructure Mesothelioma / diagnosis* Mesothelioma / ultrastructure Mesothelioma, Malignant Middle Aged Mucin-1 / analysis Mucin-1 / immunology Proto-Oncogene Proteins c-bcl-2 / analysis Proto-Oncogene Proteins c-bcl-2 / genetics* Proto-Oncogene Proteins c-bcl-2 / immunology* Real-Time Polymerase Chain Reaction Sensitivity and Specificity |
| IF | 1.985 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) ACC-MESO-4(RCB2293) |