論文 - 詳細
| RRC ID | 62790 |
|---|---|
| 著者 | Abdul Malik N, Mohamed M, Mustafa MZ, Zainuddin A. |
| タイトル | In vitro modulation of extracellular matrix genes by stingless bee honey in cellular aging of human dermal fibroblast cells. |
| ジャーナル | J Food Biochem |
| Abstract |
This study determined the antiaging effect of stingless bee honey on the expression of extracellular matrix genes. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) assay was performed for determination of optimum concentration and incubation time of stingless bee honey. Gene expression of matrix metalloproteinase-1 (MMP-1) and collagen type Ⅰ (COL1A1) were analyzed using real time reverse transcriptase polymerase chain reaction technique. Incubation with stingless bee honey at concentration of 0.02% for 72 hr showed significant increase in the viability of human fibroblast cells. Stingless bee honey significantly downregulates metalloproteinase-1 gene expression in both pre-senescence and senescence fibroblast cells and upregulates collagen type Ⅰ gene expression in senescence fibroblast cells. In conclusion, stingless bee honey potentially delayed skin aging through modulation of extracellular matrix genes. PRACTICAL APPLICATIONS: Changes of the extracellular matrix regulation promote skin aging. Stingless bee honey is a good source of natural antioxidant which potentially delays skin aging. This study demonstrated that stingless bee honey beneficially increases collagen type Ⅰ expression and decreases MMP-1 expression during cellular aging of human dermal fibroblast cells. |
| 巻・号 | 44(1) |
| ページ | e13098 |
| 公開日 | 2020-1-1 |
| DOI | 10.1111/jfbc.13098 |
| PMID | 31746481 |
| MeSH | Animals Antioxidants Bees / genetics Cellular Senescence Extracellular Matrix Fibroblasts Honey* Humans |
| IF | 1.662 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |