論文 - 詳細
| RRC ID | 62833 |
|---|---|
| 著者 | Nakaya G, Sakagami H, Koga-Ogawa Y, Shiroto A, Nobesawa T, Ueda D, Nakatani S, Kobata K, Iijima Y, Tone S, David-Gonzalez A, Garcia-Contreras R, Tomomura M, Kito S, Tamura N, Takeshima H. |
| タイトル | Augmentation of Neurotoxicity of Anticancer Drugs by X-Ray Irradiation. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND:In order to investigate the combination effect of anticancer drugs and X-ray irradiation on neurotoxic side-effects (neurotoxicity), a method that provides homogeneously X-ray-irradiated cells was newly established. MATERIALS AND METHODS:PC12 cell suspension was irradiated by X-ray (0.5 Gy) in serum-supplemented medium, immediately inoculated into 96-microwell plates and incubated overnight. The medium was replaced with fresh serum-depleted medium containing 50 ng/ml nerve growth factor to induce differentiation toward nerve-like cells with characteristic neurites according to the overlay method without changing the medium. The differentiated cells were treated by anticancer drugs as well as antioxidants, oxaliplatin or bortezomib, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. RESULTS:Antioxidants and anticancer drugs were cytotoxic to differentiating PC12 cells. Combination of anticancer drugs and X-ray irradiation slightly reduced cell viability. CONCLUSION:The present 'population irradiation method' may be useful for the investigation of the combination effect of X-ray irradiation and any pharmaceutical drug. |
| 巻・号 | 34(3) |
| ページ | 1009-1016 |
| 公開日 | 2020-1-1 |
| DOI | 10.21873/invivo.11869 |
| PII | 34/3/1009 |
| PMID | 32354886 |
| PMC | PMC7279790 |
| MeSH | Animals Antineoplastic Agents / adverse effects* Antineoplastic Agents / therapeutic use Apoptosis / drug effects Biomarkers Cell Line, Tumor Cell Survival / drug effects Disease Models, Animal Immunohistochemistry Mice Nervous System / drug effects* Phosphorylation Radiation, Ionizing* Signal Transduction / drug effects X-Rays* |
| IF | 1.541 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-2(RCB1945) HSC-3(RCB1975) HSC-4(RCB1902) |