| RRC ID |
62839
|
| Author |
Matsuzaki M, Mandai M, Yamanari M, Totani K, Nishida M, Sugita S, Maeda T, Koide N, Takagi S, Hirami Y, Miyamoto N, Sugiyama S, Takahashi M, Kurimoto Y.
|
| Title |
Polarization-sensitive optical coherence tomography for estimating relative melanin content of autologous induced stem-cell derived retinal pigment epithelium.
|
| Journal |
Sci Rep
|
| Abstract |
Transplantation of autologous human induced pluripotent stem cell-derived retinal pigment epithelial (hiPSC-RPE) sheets is a promising therapy for age-related macular degeneration (AMD). As melanin content is a representative feature of healthy RPE, we used polarization-sensitive optical coherence tomography (PS-OCT) to estimate the relative melanin content of RPE in diseased and non-diseased area, and in human iPSC-RPE sheets in vitro and in vivo by evaluating the randomness of polarization (entropy). Two aged Japanese women, one with neovascular AMD that underwent transplantation of an autologous hiPSC-RPE cell sheet and another with binocular dry AMD, were selected for this study. Entropy value was minimal in cells containing no melanin, whereas that of human RPE and hiPSC-RPE sheets was high. En face entropy of the cultured hiPSC-RPE sheet was compared with its grey-scale photo and its values were found to be inversely correlated with the extent of absence of pigmentation in vitro. En face entropy maps were compared to colour fundus photographs, fundus autofluorescence images, and fluorescein angiography images from patients. Entropy values of intact and defective RPEs and of iPSC-RPE transplant areas were determined in vivo using PS-OCT B-scan images. PS-OCT was found to be applicable in the estimation of relative melanin content of cultured and transplanted RPEs in regenerative medicine.
|
| Volume |
10(1)
|
| Pages |
7656
|
| Published |
2020-5-6
|
| DOI |
10.1038/s41598-020-64601-4
|
| PII |
10.1038/s41598-020-64601-4
|
| PMID |
32376945
|
| PMC |
PMC7203130
|
| MeSH |
Aged
Aged, 80 and over
Biomarkers*
Cell Culture Techniques
Cell Differentiation
Female
Fluorescein Angiography
HEK293 Cells
Humans
Induced Pluripotent Stem Cells / cytology*
Macular Degeneration / diagnostic imaging
Macular Degeneration / etiology
Macular Degeneration / metabolism
Macular Degeneration / pathology
Male
Melanins / metabolism*
Retinal Pigment Epithelium / cytology
Retinal Pigment Epithelium / diagnostic imaging*
Retinal Pigment Epithelium / metabolism*
Tomography, Optical Coherence* / methods
|
| IF |
3.998
|
| Resource |
| Human and Animal Cells |
293T(RCB2202) |
