論文 - 詳細
| RRC ID | 62856 |
|---|---|
| 著者 | Tanaka M, Sato A, Kishimoto Y, Mabashi-Asazuma H, Kondo K, Iida K. |
| タイトル | Gallic Acid Inhibits Lipid Accumulation via AMPK Pathway and Suppresses Apoptosis and Macrophage-Mediated Inflammation in Hepatocytes. |
| ジャーナル | Nutrients |
| Abstract |
Nonalcoholic fatty liver disease (NAFLD) is one of the most common causes of chronic liver disease, sometimes ranges from simple steatosis to nonalcoholic steatohepatitis (NASH). Various hits including excessive hepatic steatosis, oxidative stress, apoptosis, and inflammation, contribute to NASH development. Gallic acid (GA), a natural polyphenol, was reported to exert a protective effect on hepatic steatosis in animal models, but the precise molecular mechanisms remain unclear. Here, we examined the effect of GA on hepatic lipid accumulation, apoptosis, and inflammatory response caused by hepatocyte-macrophage crosstalk. We demonstrated that GA attenuated palmitic acid (PA)-induced fat accumulation via the activation of AMP-activated protein kinase (AMPK) in HepG2 cells. GA also ameliorated cell viability and suppressed apoptosis-related gene expression and caspase 3/7 activity induced by PA and H2O2. In a co-culture of lipid-laden Hepa 1-6 hepatocytes and RAW 264 macrophages, GA reduced inflammatory mediator expression and induced antioxidant enzyme expression. These results indicate that GA suppresses hepatic lipid accumulation, apoptosis, and inflammation caused by the interaction between hepatocytes and macrophages. The potential effects of GA observed in our study could be effective in preventing NASH and its complications. |
| 巻・号 | 12(5) |
| 公開日 | 2020-5-20 |
| DOI | 10.3390/nu12051479 |
| PII | nu12051479 |
| PMID | 32443660 |
| PMC | PMC7285059 |
| MeSH | AMP-Activated Protein Kinases / metabolism* Animals Apoptosis / drug effects* Caspase 3 / metabolism Caspase 7 / metabolism Gallic Acid / pharmacology* Gene Expression / drug effects Hep G2 Cells Hepatocytes / drug effects* Humans Hydrogen Peroxide Inflammation Inflammation Mediators / metabolism* Lipid Metabolism / drug effects* Lipids Liver / metabolism Macrophages / drug effects* Mice Non-alcoholic Fatty Liver Disease / etiology Oxidative Stress / drug effects Palmitic Acid / metabolism Polyphenols |
| IF | 4.546 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) Hepa 1-6(RCB1638) Hep G2(RCB1886) |