論文 - 詳細
| RRC ID | 6289 |
|---|---|
| 著者 | Peng L, Fukao Y, Fujiwara M, Takami T, Shikanai T. |
| タイトル | Efficient operation of NAD(P)H dehydrogenase requires supercomplex formation with photosystem I via minor LHCI in Arabidopsis. |
| ジャーナル | Plant Cell |
| Abstract |
In higher plants, the chloroplast NAD(P)H dehydrogenase (NDH) complex mediates photosystem I (PSI) cyclic and chlororespiratory electron transport. We reported previously that NDH interacts with the PSI complex to form a supercomplex (NDH-PSI). In this study, NDH18 and FKBP16-2 (FK506 Binding Protein 16-2), detected in the NDH-PSI supercomplex by mass spectrometry, were shown to be NDH subunits by the analysis of their knockdown lines. On the basis of extensive mutant characterization, we propose a structural model for chloroplast NDH, whereby NDH is divided into four subcomplexes. The subcomplex A and membrane subcomplex are conserved in cyanobacteria, but the subcomplex B and lumen subcomplex are specific to chloroplasts. Two minor light-harvesting complex I proteins, Lhca5 and Lhca6, were required for the full-size NDH-PSI supercomplex formation. Similar to crr pgr5 double mutants that completely lack cyclic electron flow activity around PSI, the lhca6 pgr5 double mutant exhibited a severe defect in growth. Consistent with the impaired NDH activity, photosynthesis was also severely affected in mature leaves of lhca6 pgr5. We conclude that chloroplast NDH became equipped with the novel subcomplexes and became associated with PSI during the evolution of land plants, and this process may have facilitated the efficient operation of NDH. |
| 巻・号 | 21(11) |
| ページ | 3623-40 |
| 公開日 | 2009-11-1 |
| DOI | 10.1105/tpc.109.068791 |
| PII | tpc.109.068791 |
| PMID | 19903870 |
| PMC | PMC2798312 |
| MeSH | Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Chloroplasts / genetics Chloroplasts / metabolism* Evolution, Molecular Light-Harvesting Protein Complexes / genetics Light-Harvesting Protein Complexes / metabolism* Macromolecular Substances / metabolism NADPH Dehydrogenase / metabolism* Photosynthesis / physiology* Photosynthetic Reaction Center Complex Proteins / genetics Photosynthetic Reaction Center Complex Proteins / metabolism Photosystem I Protein Complex / genetics Photosystem I Protein Complex / metabolism* |
| IF | 9.618 |
| 引用数 | 156 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst15064 |