論文 - 詳細
| RRC ID | 62956 |
|---|---|
| 著者 | Bito T, Koseki K, Asano R, Ueda N, Yamada T, Yabuta Y, Ichiyanagi T, Ishihara A, Watanabe K, Watanabe F. |
| タイトル | 5-hydroxymethyl-2-furaldehyde purified from Japanese pear (Pyrus pyrifolia Nakai cv. Nijisseiki) juice concentrate inhibits melanogenesis in B16 mouse melanoma cells. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Pear juice concentrate prepared by boiling Japanese pear (Pyrus pyrifolia Nakai cv. Nijisseiki) juice can significantly inhibit the activity of tyrosinase, a key enzyme in melanin synthesis in human skin. Using the ethanol extract of pear juice concentrate, we homogeneously purified an active compound that was identified as 5-hydroxymethyl-2-furaldehyde (5-HMF) through 1H- and 13C-NMR and mass spectroscopy. We observed that 5-HMF inhibited the monophenolase and diphenolase activities of mushroom tyrosinase as a mixed-type inhibitor (Ki values of 3.81 and 3.70 mmol/L, respectively). In B16 mouse melanoma cells, treatment with 170 µmol/L of 5-HMF significantly reduced α-melanocyte-stimulated melanin synthesis by suppressing the cyclic adenosine monophosphate-dependent signaling pathway involved in melanogenesis. The results of our study indicated that 5-HMF can be potentially used as a skin-lightening agent in the cosmetic industry. Abbreviations: AC: adenylate cyclase; CREB: cAMP response element-binding protein; dhFAME: S-(-)-10,11-Dihydroxyfarnesoic acid methyl ester; DMEM: dulbecco's modified eagle medium; l-DOPA: 3-(3,4-Dihydroxyphenyl)- l-alanine; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HEPES: 4-(2-Hydroxyethyl)-1-piperazine ethane sulfonic acid; 5-HMF: 5-Hydroxymethyl-2-furaldehyde; MITF: microphthalmia-associated transcription factor; α-MSH: α-Melanocyte-stimulating hormone; PKA: protein kinase A; PVDF: polyvinylidene difluoride; SDS: sodium dodecyl sulfate; TRP1: tyrosinase-related protein 1; TRP2: tyrosinase-related protein 2. |
| 巻・号 | 84(11) |
| ページ | 2374-2384 |
| 公開日 | 2020-11-1 |
| DOI | 10.1080/09168451.2020.1792762 |
| PMID | 32674671 |
| MeSH | Animals Cell Line, Tumor Fruit and Vegetable Juices / analysis* Furaldehyde / analogs & derivatives* Furaldehyde / isolation & purification Furaldehyde / pharmacology Melanins / biosynthesis* Melanoma, Experimental / pathology* Mice Monophenol Monooxygenase / antagonists & inhibitors Oxidoreductases / antagonists & inhibitors Pyrus / chemistry* |
| IF | 1.516 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma(RCB1283) |