| RRC ID |
62989
|
| 著者 |
Suzuki K, Matsumoto M, Katoh Y, Liu L, Ochiai K, Aizawa Y, Nagatomi R, Okuno H, Itoi E, Igarashi K.
|
| タイトル |
Bach1 promotes muscle regeneration through repressing Smad-mediated inhibition of myoblast differentiation.
|
| ジャーナル |
PLoS One
|
| Abstract |
It has been reported that Bach1-deficient mice show reduced tissue injuries in diverse disease models due to increased expression of heme oxygenase-1 (HO-1)that possesses an antioxidant function. In contrast, we found that Bach1 deficiency in mice exacerbated skeletal muscle injury induced by cardiotoxin. Inhibition of Bach1 expression in C2C12 myoblast cells using RNA interference resulted in reduced proliferation, myotube formation, and myogenin expression compared with control cells. While the expression of HO-1 was increased by Bach1 silencing in C2C12 cells, the reduced myotube formation was not rescued by HO-1 inhibition. Up-regulations of Smad2, Smad3 and FoxO1, known inhibitors of muscle cell differentiation, were observed in Bach1-deficient mice and Bach1-silenced C2C12 cells. Therefore, Bach1 may promote regeneration of muscle by increasing proliferation and differentiation of myoblasts.
|
| 巻・号 |
15(8)
|
| ページ |
e0236781
|
| 公開日 |
2020-1-1
|
| DOI |
10.1371/journal.pone.0236781
|
| PII |
PONE-D-19-14231
|
| PMID |
32776961
|
| PMC |
PMC7416950
|
| MeSH |
Animals
Basic-Leucine Zipper Transcription Factors / deficiency
Basic-Leucine Zipper Transcription Factors / genetics
Basic-Leucine Zipper Transcription Factors / metabolism*
Cell Differentiation*
Cell Line
Gene Knockdown Techniques
Gene Silencing
Mice
Muscle, Skeletal / cytology
Muscle, Skeletal / physiology*
Myoblasts / cytology*
Regeneration*
Smad Proteins / metabolism*
Transcriptome / genetics
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
