RRC ID 63018
著者 Lin YY, Sun D, Wu YL.
タイトル Novel regulation of PKC-induced inflammation by Akt and protein phosphatase 2A in ovarian granulosa cells.
ジャーナル Chin J Physiol
Abstract PKC-mediated inflammation is important in ovarian physiology. The roles of Akt and protein phosphatase 2A (PP2A) in PKC-mediated inflammation in ovarian granulosa cells (GCs) remain mostly unclear. PKC activator phorbol 12-myristate 13-acetate induced the Akt phosphorylation in rat primary GCs but reduced the Akt phosphorylation in KGN human GCs. In rat GCs, an inhibitory effect of PI3K inhibitor wortmannin and a stimulatory effect of Akt activator SC79 on PKC-induced cyclooxygenase-2 (COX-2)/PGE2production were noted; wortmannin and SC79 acted oppositely in human GCs. In rat GCs, PP2A inhibitor okadaic acid further enhanced the PKC-mediated promoter activation and elevation of mRNA and protein levels of the COX-2 gene, whereas PP2A activator sodium selenate attenuated the PKC-mediated COX-2 expression and promoter activation. PKC activation did not affect PP2A phosphorylation, but okadaic acid indeed augmented the PKC-induced NF-κB nuclear translocation. Thus, PP2A appears to act as a negative modulator in PKC-mediated cellular inflammation in rat GCs, at least in part due to its attenuating effect on the PKC-induced NF-κB activation.
巻・号 63(4)
ページ 179-186
公開日 2020-1-1
DOI 10.4103/CJP.CJP_44_20
PII ChinJPhysiol_2020_63_4_179_293586
PMID 32859885
MeSH Animals Female Granulosa Cells* Humans Inflammation Phosphatidylinositol 3-Kinases Phosphorylation Protein Phosphatase 2 Proto-Oncogene Proteins c-akt Rats
IF 1.151
リソース情報
ヒト・動物細胞 KGN(RCB1154)