RRC ID 6304
Author Nakashima K, Tran LS, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K.
Title Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice.
Journal Plant J
Abstract The OsNAC6 gene is a member of the NAC transcription factor gene family in rice. Expression of OsNAC6 is induced by abiotic stresses, including cold, drought and high salinity. OsNAC6 gene expression is also induced by wounding and blast disease. A transactivation assay using a yeast system demonstrated that OsNAC6 functions as a transcriptional activator, and transient localization studies with OsNAC6-sGFP fusion protein revealed its nuclear localization. Transgenic rice plants over-expressing OsNAC6 constitutively exhibited growth retardation and low reproductive yields. These transgenic rice plants showed an improved tolerance to dehydration and high-salt stresses, and also exhibited increased tolerance to blast disease. By utilizing stress-inducible promoters, such as the OsNAC6 promoter, it is hoped that stress-inducible over-expression of OsNAC6 in rice can improve stress tolerance by suppressing the negative effects of OsNAC6 on growth under normal growth conditions. The results of microarray analysis revealed that many genes that are inducible by abiotic and biotic stresses were upregulated in rice plants over-expressing OsNAC6. A transient transactivation assay showed that OsNAC6 activates the expression of at least two genes, including a gene encoding peroxidase. Collectively, these results indicate that OsNAC6 functions as a transcriptional activator in response to abiotic and biotic stresses in plants. We conclude that OsNAC6 may serve as a useful biotechnological tool for the improvement of stress tolerance in various kinds of plants.
Volume 51(4)
Pages 617-30
Published 2007-8-1
DOI 10.1111/j.1365-313X.2007.03168.x
PMID 17587305
MeSH Cold Temperature Disasters Gene Expression Profiling* Gene Expression Regulation, Plant / drug effects Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Oligonucleotide Array Sequence Analysis Oryza / genetics* Oryza / growth & development Oryza / metabolism Plant Proteins / genetics* Plant Proteins / metabolism Plant Proteins / physiology Plants, Genetically Modified RNA, Messenger / genetics RNA, Messenger / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sodium Chloride / pharmacology Transcription Factors / genetics* Transcription Factors / metabolism Transcription Factors / physiology Transcription, Genetic / drug effects
IF 5.726
Times Cited 566
Arabidopsis / Cultured plant cells, genes rpc00031