| RRC ID |
6304
|
| Author |
Nakashima K, Tran LS, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K.
|
| Title |
Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice.
|
| Journal |
Plant J
|
| Abstract |
The OsNAC6 gene is a member of the NAC transcription factor gene family in rice. Expression of OsNAC6 is induced by abiotic stresses, including cold, drought and high salinity. OsNAC6 gene expression is also induced by wounding and blast disease. A transactivation assay using a yeast system demonstrated that OsNAC6 functions as a transcriptional activator, and transient localization studies with OsNAC6-sGFP fusion protein revealed its nuclear localization. Transgenic rice plants over-expressing OsNAC6 constitutively exhibited growth retardation and low reproductive yields. These transgenic rice plants showed an improved tolerance to dehydration and high-salt stresses, and also exhibited increased tolerance to blast disease. By utilizing stress-inducible promoters, such as the OsNAC6 promoter, it is hoped that stress-inducible over-expression of OsNAC6 in rice can improve stress tolerance by suppressing the negative effects of OsNAC6 on growth under normal growth conditions. The results of microarray analysis revealed that many genes that are inducible by abiotic and biotic stresses were upregulated in rice plants over-expressing OsNAC6. A transient transactivation assay showed that OsNAC6 activates the expression of at least two genes, including a gene encoding peroxidase. Collectively, these results indicate that OsNAC6 functions as a transcriptional activator in response to abiotic and biotic stresses in plants. We conclude that OsNAC6 may serve as a useful biotechnological tool for the improvement of stress tolerance in various kinds of plants.
|
| Volume |
51(4)
|
| Pages |
617-30
|
| Published |
2007-8-1
|
| DOI |
10.1111/j.1365-313X.2007.03168.x
|
| PII |
TPJ3168
|
| PMID |
17587305
|
| MeSH |
Cold Temperature
Disasters
Gene Expression Profiling*
Gene Expression Regulation, Plant / drug effects
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Oligonucleotide Array Sequence Analysis
Oryza / genetics*
Oryza / growth & development
Oryza / metabolism
Plant Proteins / genetics*
Plant Proteins / metabolism
Plant Proteins / physiology
Plants, Genetically Modified
RNA, Messenger / genetics
RNA, Messenger / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Sodium Chloride / pharmacology
Transcription Factors / genetics*
Transcription Factors / metabolism
Transcription Factors / physiology
Transcription, Genetic / drug effects
|
| IF |
6.141
|
| Times Cited |
566
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00031 |
