論文 - 詳細
| RRC ID | 6304 |
|---|---|
| 著者 | Nakashima K, Tran LS, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K. |
| タイトル | Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice. |
| ジャーナル | Plant J |
| Abstract |
The OsNAC6 gene is a member of the NAC transcription factor gene family in rice. Expression of OsNAC6 is induced by abiotic stresses, including cold, drought and high salinity. OsNAC6 gene expression is also induced by wounding and blast disease. A transactivation assay using a yeast system demonstrated that OsNAC6 functions as a transcriptional activator, and transient localization studies with OsNAC6-sGFP fusion protein revealed its nuclear localization. Transgenic rice plants over-expressing OsNAC6 constitutively exhibited growth retardation and low reproductive yields. These transgenic rice plants showed an improved tolerance to dehydration and high-salt stresses, and also exhibited increased tolerance to blast disease. By utilizing stress-inducible promoters, such as the OsNAC6 promoter, it is hoped that stress-inducible over-expression of OsNAC6 in rice can improve stress tolerance by suppressing the negative effects of OsNAC6 on growth under normal growth conditions. The results of microarray analysis revealed that many genes that are inducible by abiotic and biotic stresses were upregulated in rice plants over-expressing OsNAC6. A transient transactivation assay showed that OsNAC6 activates the expression of at least two genes, including a gene encoding peroxidase. Collectively, these results indicate that OsNAC6 functions as a transcriptional activator in response to abiotic and biotic stresses in plants. We conclude that OsNAC6 may serve as a useful biotechnological tool for the improvement of stress tolerance in various kinds of plants. |
| 巻・号 | 51(4) |
| ページ | 617-30 |
| 公開日 | 2007-8-1 |
| DOI | 10.1111/j.1365-313X.2007.03168.x |
| PII | TPJ3168 |
| PMID | 17587305 |
| MeSH | Cold Temperature Disasters Gene Expression Profiling* Gene Expression Regulation, Plant / drug effects Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Oligonucleotide Array Sequence Analysis Oryza / genetics* Oryza / growth & development Oryza / metabolism Plant Proteins / genetics* Plant Proteins / metabolism Plant Proteins / physiology Plants, Genetically Modified RNA, Messenger / genetics RNA, Messenger / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sodium Chloride / pharmacology Transcription Factors / genetics* Transcription Factors / metabolism Transcription Factors / physiology Transcription, Genetic / drug effects |
| IF | 6.141 |
| 引用数 | 566 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00031 |