論文 - 詳細
| RRC ID | 63089 |
|---|---|
| 著者 | Sakamoto Y, Kokuta T, Teshigahara A, Iijima K, Kitao H, Takata M, Tauchi H. |
| タイトル | Mitotic cells can repair DNA double-strand breaks via a homology-directed pathway. |
| ジャーナル | J Radiat Res |
| Abstract |
The choice of repair pathways of DNA double-strand breaks (DSBs) is dependent upon the cell cycle phases. While homologous recombination repair (HRR) is active between the S and G2 phases, its involvement in mitotic DSB repair has not been examined in detail. In the present study, we developed a new reporter assay system to detect homology-directed repair (HDR), a major pathway used for HRR, in combination with an inducible DSB-generation system. As expected, the maximal HDR activity was observed in the late S phase, along with minimal activity in the G1 phase and at the G1/S boundary. Surprisingly, significant HDR activity was observed in M phase, and the repair efficiency was similar to that observed in late S phase. HDR was also confirmed in metaphase cells collected with continuous colcemid exposure. ChIP assays revealed the recruitment of RAD51 to the vicinity of DSBs in M phase. In addition, the ChIP assay for gamma-H2AX and phosphorylated DNA-PKcs indicated that a part of M-phase cells with DSBs could proceed into the next G1 phase. These results provide evidence showing that a portion of mitotic cell DSBs are undoubtedly repaired through action of the HDR repair pathway. |
| 巻・号 | 62(1) |
| ページ | 25-33 |
| 公開日 | 2021-1-1 |
| DOI | 10.1093/jrr/rraa095 |
| PII | 5917391 |
| PMID | 33009557 |
| PMC | PMC7779344 |
| MeSH | Cell Line Chromatin Immunoprecipitation DNA Breaks, Double-Stranded* Humans Kinetics Mitosis* Real-Time Polymerase Chain Reaction Recombinational DNA Repair* |
| IF | 1.95 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MRC-5 SV1 TG1(RCB0207) |