RRC ID 63178
著者 Yamabe Y, Kawagoe Y, Okuno K, Inoue M, Chikaoka K, Ueda D, Tajima Y, Yamada TK, Kakihara Y, Hara T, Sato T.
タイトル Construction of an artificial system for ambrein biosynthesis and investigation of some biological activities of ambrein.
ジャーナル Sci Rep
Abstract Ambergris, a sperm whale metabolite, has long been used as a fragrance and traditional medication, but it is now rarely available. The odor components of ambergris result from the photooxidative degradation of the major component, ambrein. The pharmacological activities of ambergris have also been attributed to ambrein. However, efficient production of ambrein and odor compounds has not been achieved. Here, we constructed a system for the synthesis of ambrein and odor components. First, we created a new triterpene synthase, "ambrein synthase," for mass production of ambrein by redesigning a bacterial enzyme. The ambrein yields were approximately 20 times greater than those reported previously. Next, an efficient photooxidative conversion system from ambrein to a range of volatiles of ambergris was established. The yield of volatiles was 8-15%. Finally, two biological activities, promotion of osteoclast differentiation and prevention of amyloid β-induced apoptosis, were discovered using the synthesized ambrein.
巻・号 10(1)
ページ 19643
公開日 2020-11-12
DOI 10.1038/s41598-020-76624-y
PII 10.1038/s41598-020-76624-y
PMID 33184314
PMC PMC7661701
MeSH Ambergris / chemistry* Amyloid beta-Peptides / pharmacology Animals Apoptosis* Cell Differentiation Cell Line Humans Naphthols / chemistry* Naphthols / pharmacology* Osteoclasts / cytology* Osteoclasts / drug effects Osteoclasts / metabolism Sperm Whale / metabolism* Triterpenes / chemistry Triterpenes / pharmacology Volatile Organic Compounds / chemistry Volatile Organic Compounds / pharmacology
IF 3.998
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)