論文 - 詳細
| RRC ID | 63242 |
|---|---|
| 著者 | Fujiwara Y, Murofushi T, Koshi R, Mikami Y, Tsuda H. |
| タイトル | Reactive oxygen species-dependent release of damage-associated molecular patterns from human gingival epithelial Ca9-22 cells during butyrate or propionate exposure. |
| ジャーナル | J Oral Sci |
| Abstract |
Treating the gingival epithelial Ca9-22 cell with butyrate, a short-chain fatty acid (SCFA) produced by bacteria within mature dental plaque, induces necrotic cellular death. In this report, it was examined whether SCFA-mediated cellular death is accompanied by a release of damage-associated molecular patterns (DAMPs). In addition, the role of reactive oxygen species (ROS) in the release of DAMPs was evaluated. Human gingival epithelial Ca9-22 cells were treated with butyrate or propionate. The amounts of dead cells were then measured using SYTOX-green dye. Released DAMPs were detected by western blot. The role of ROS scavengers, ascorbic acid and N-acetylcysteine, on DAMP-release was evaluated. Dose and time-dependent induction of Ca9-22 cell death was observed during butyrate and propionate treatments. This was accompanied by the release of DAMPs. Ascorbic acid or N-acetylcysteine reduced cellular death and inhibited DAMP-release induced by exposure to butyrate or propionate. These data collectively suggest that SCFA-induced death of gingival epithelial Ca9-22 cells and accompanying release of DAMPs are dependent on ROS. |
| 巻・号 | 63(2) |
| ページ | 195-197 |
| 公開日 | 2021-3-31 |
| DOI | 10.2334/josnusd.20-0411 |
| PMID | 33390461 |
| MeSH | Antigens, Neoplasm Butyrates* / pharmacology Carbonic Anhydrase IX Epithelial Cells Gingiva Humans Propionates* / pharmacology Reactive Oxygen Species |
| IF | 1.2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) |