| RRC ID |
63246
|
| 著者 |
Park E, Lee CG, Lim E, Hwang S, Yun SH, Kim J, Jeong H, Yong Y, Yun SH, Choi CW, Jin HS, Jeong SY.
|
| タイトル |
Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and gastrointestinal motility, as well as on arthritis. Here, we fractionated and isolated bioactive constituent(s) responsible for anti-osteoporotic effects of GL root extract. A single phytochemical compound, loganic acid, was identified as a candidate osteoprotective agent. Its anti-osteoporotic effects were examined in vitro and in vivo. Treatment with loganic acid significantly increased osteoblastic differentiation in preosteoblast MC3T3-E1 cells by promoting alkaline phosphatase activity and increasing mRNA expression levels of bone metabolic markers such as Alpl, Bglap, and Sp7. However, loganic acid inhibited osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. For in vivo experiments, the effect of loganic acid on ovariectomized (OVX) mice was examined for 12 weeks. Loganic acid prevented OVX-induced bone mineral density loss and improved bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for treatment of osteoporosis.
|
| 巻・号 |
22(1)
|
| 公開日 |
2020-12-28
|
| DOI |
10.3390/ijms22010233
|
| PII |
ijms22010233
|
| PMID |
33379387
|
| PMC |
PMC7795511
|
| MeSH |
Administration, Oral
Animals
Carbon-13 Magnetic Resonance Spectroscopy
Cell Differentiation / drug effects
Cell Line
Cells, Cultured
Disease Models, Animal
Gentiana / chemistry
Iridoids / administration & dosage
Iridoids / chemistry
Iridoids / isolation & purification
Iridoids / pharmacology*
Mice
Osteoblasts / drug effects*
Osteoblasts / pathology
Osteoclasts / drug effects*
Osteoclasts / pathology
Osteoporosis / pathology*
Ovariectomy
Plant Extracts / pharmacology
Plant Roots / chemistry
Protective Agents / pharmacology*
Proton Magnetic Resonance Spectroscopy
|
| IF |
4.556
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
