RRC ID 63246
Author Park E, Lee CG, Lim E, Hwang S, Yun SH, Kim J, Jeong H, Yong Y, Yun SH, Choi CW, Jin HS, Jeong SY.
Title Osteoprotective Effects of Loganic Acid on Osteoblastic and Osteoclastic Cells and Osteoporosis-Induced Mice.
Journal Int J Mol Sci
Abstract Osteoporosis is a common disease caused by an imbalance of processes between bone resorption by osteoclasts and bone formation by osteoblasts in postmenopausal women. The roots of Gentiana lutea L. (GL) are reported to have beneficial effects on various human diseases related to liver functions and gastrointestinal motility, as well as on arthritis. Here, we fractionated and isolated bioactive constituent(s) responsible for anti-osteoporotic effects of GL root extract. A single phytochemical compound, loganic acid, was identified as a candidate osteoprotective agent. Its anti-osteoporotic effects were examined in vitro and in vivo. Treatment with loganic acid significantly increased osteoblastic differentiation in preosteoblast MC3T3-E1 cells by promoting alkaline phosphatase activity and increasing mRNA expression levels of bone metabolic markers such as Alpl, Bglap, and Sp7. However, loganic acid inhibited osteoclast differentiation of primary-cultured monocytes derived from mouse bone marrow. For in vivo experiments, the effect of loganic acid on ovariectomized (OVX) mice was examined for 12 weeks. Loganic acid prevented OVX-induced bone mineral density loss and improved bone structural properties in osteoporotic model mice. These results suggest that loganic acid may be a potential therapeutic candidate for treatment of osteoporosis.
Volume 22(1)
Published 2020-12-28
DOI 10.3390/ijms22010233
PII ijms22010233
PMID 33379387
PMC PMC7795511
MeSH Administration, Oral Animals Carbon-13 Magnetic Resonance Spectroscopy Cell Differentiation / drug effects Cell Line Cells, Cultured Disease Models, Animal Gentiana / chemistry Iridoids / administration & dosage Iridoids / chemistry Iridoids / isolation & purification Iridoids / pharmacology* Mice Osteoblasts / drug effects* Osteoblasts / pathology Osteoclasts / drug effects* Osteoclasts / pathology Osteoporosis / pathology* Ovariectomy Plant Extracts / pharmacology Plant Roots / chemistry Protective Agents / pharmacology* Proton Magnetic Resonance Spectroscopy
IF 4.556
Human and Animal Cells MC3T3-E1(RCB1126)