| RRC ID |
63264
|
| Author |
Miyashita S, Sagane Y, Inui K, Hayashi S, Miyata K, Suzuki T, Ohyama T, Watanabe T, Niwa K.
|
| Title |
Botulinum toxin complex increases paracellular permeability in intestinal epithelial cells via activation of p38 mitogen-activated protein kinase.
|
| Journal |
J Vet Med Sci
|
| Abstract |
Clostridium botulinum produces a large toxin complex (L-TC) that increases paracellular permeability in intestinal epithelial cells by a mechanism that remains unclear. Here, we show that mitogen-activated protein kinases (MAPKs) are involved in this permeability increase. Paracellular permeability was measured by FITC-dextran flux through a monolayer of rat intestinal epithelial IEC-6 cells, and MAPK activation was estimated from western blots. L-TC of C. botulinum serotype D strain 4947 increased paracellular dextran flux and activated extracellular signal-regulated kinase (ERK), p38, but not c-Jun N-terminal kinase (JNK) in IEC-6 cells. The permeability increase induced by L-TC was abrogated by the p38 inhibitor SB203580. These results indicate that L-TC increases paracellular permeability by activating p38, but not JNK and ERK.
|
| Volume |
75(12)
|
| Pages |
1637-42
|
| Published |
2013-12-30
|
| DOI |
10.1292/jvms.13-0164
|
| PII |
DN/JST.JSTAGE/jvms/13-0164
|
| PMID |
23884081
|
| PMC |
PMC3942962
|
| MeSH |
Animals
Blotting, Western
Botulinum Toxins / pharmacokinetics
Botulinum Toxins / toxicity*
Cell Line
Dextrans
Enzyme Activation / drug effects*
Fluorescein-5-isothiocyanate / analogs & derivatives
Intestinal Mucosa / drug effects*
Permeability / drug effects
Rats
Signal Transduction / drug effects*
p38 Mitogen-Activated Protein Kinases / metabolism*
|
| IF |
1.049
|
| Resource |
| Human and Animal Cells |
IEC 6(RCB0993) |
