RRC ID 63264
著者 Miyashita S, Sagane Y, Inui K, Hayashi S, Miyata K, Suzuki T, Ohyama T, Watanabe T, Niwa K.
タイトル Botulinum toxin complex increases paracellular permeability in intestinal epithelial cells via activation of p38 mitogen-activated protein kinase.
ジャーナル J Vet Med Sci
Abstract Clostridium botulinum produces a large toxin complex (L-TC) that increases paracellular permeability in intestinal epithelial cells by a mechanism that remains unclear. Here, we show that mitogen-activated protein kinases (MAPKs) are involved in this permeability increase. Paracellular permeability was measured by FITC-dextran flux through a monolayer of rat intestinal epithelial IEC-6 cells, and MAPK activation was estimated from western blots. L-TC of C. botulinum serotype D strain 4947 increased paracellular dextran flux and activated extracellular signal-regulated kinase (ERK), p38, but not c-Jun N-terminal kinase (JNK) in IEC-6 cells. The permeability increase induced by L-TC was abrogated by the p38 inhibitor SB203580. These results indicate that L-TC increases paracellular permeability by activating p38, but not JNK and ERK.
巻・号 75(12)
ページ 1637-42
公開日 2013-12-30
DOI 10.1292/jvms.13-0164
PII DN/JST.JSTAGE/jvms/13-0164
PMID 23884081
PMC PMC3942962
MeSH Animals Blotting, Western Botulinum Toxins / pharmacokinetics Botulinum Toxins / toxicity* Cell Line Dextrans Enzyme Activation / drug effects* Fluorescein-5-isothiocyanate / analogs & derivatives Intestinal Mucosa / drug effects* Permeability / drug effects Rats Signal Transduction / drug effects* p38 Mitogen-Activated Protein Kinases / metabolism*
IF 1.049
リソース情報
ヒト・動物細胞 IEC 6(RCB0993)