論文 - 詳細
| RRC ID | 63264 |
|---|---|
| 著者 | Miyashita S, Sagane Y, Inui K, Hayashi S, Miyata K, Suzuki T, Ohyama T, Watanabe T, Niwa K. |
| タイトル | Botulinum toxin complex increases paracellular permeability in intestinal epithelial cells via activation of p38 mitogen-activated protein kinase. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Clostridium botulinum produces a large toxin complex (L-TC) that increases paracellular permeability in intestinal epithelial cells by a mechanism that remains unclear. Here, we show that mitogen-activated protein kinases (MAPKs) are involved in this permeability increase. Paracellular permeability was measured by FITC-dextran flux through a monolayer of rat intestinal epithelial IEC-6 cells, and MAPK activation was estimated from western blots. L-TC of C. botulinum serotype D strain 4947 increased paracellular dextran flux and activated extracellular signal-regulated kinase (ERK), p38, but not c-Jun N-terminal kinase (JNK) in IEC-6 cells. The permeability increase induced by L-TC was abrogated by the p38 inhibitor SB203580. These results indicate that L-TC increases paracellular permeability by activating p38, but not JNK and ERK. |
| 巻・号 | 75(12) |
| ページ | 1637-42 |
| 公開日 | 2013-12-30 |
| DOI | 10.1292/jvms.13-0164 |
| PII | DN/JST.JSTAGE/jvms/13-0164 |
| PMID | 23884081 |
| PMC | PMC3942962 |
| MeSH | Animals Blotting, Western Botulinum Toxins / pharmacokinetics Botulinum Toxins / toxicity* Cell Line Dextrans Enzyme Activation / drug effects* Fluorescein-5-isothiocyanate / analogs & derivatives Intestinal Mucosa / drug effects* Permeability / drug effects Rats Signal Transduction / drug effects* p38 Mitogen-Activated Protein Kinases / metabolism* |
| IF | 1.049 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IEC 6(RCB0993) |