論文 - 詳細
| RRC ID | 63266 |
|---|---|
| 著者 | Uno K, Sugimoto N, Sato Y. |
| タイトル | N-aryl pyrido cyanine derivatives are nuclear and organelle DNA markers for two-photon and super-resolution imaging. |
| ジャーナル | Nat Commun |
| Abstract |
Live cell imaging using fluorescent DNA markers are an indispensable molecular tool in various biological and biomedical fields. It is a challenge to develop DNA probes that avoid UV light photo-excitation, have high specificity for DNA, are cell-permeable and are compatible with cutting-edge imaging techniques such as super-resolution microscopy. Herein, we present N-aryl pyrido cyanine (N-aryl-PC) derivatives as a class of long absorption DNA markers with absorption in the wide range of visible light. The high DNA specificity and membrane permeability allow the staining of both organelle DNA as well as nuclear DNA, in various cell types, including plant tissues, without the need for washing post-staining. N-aryl-PC dyes are also highly compatible with a separation of photon by lifetime tuning method in stimulated emission depletion microscopy (SPLIT-STED) for super-resolution imaging as well as two-photon microscopy for deep tissue imaging, making it a powerful tool in the life sciences. |
| 巻・号 | 12(1) |
| ページ | 2650 |
| 公開日 | 2021-5-11 |
| DOI | 10.1038/s41467-021-23019-w |
| PII | 10.1038/s41467-021-23019-w |
| PMID | 33976192 |
| PMC | PMC8113587 |
| MeSH | Animals Arabidopsis / cytology Benzimidazoles / chemistry Cell Line, Tumor Cell Nucleus / chemistry* Cell Nucleus / metabolism Cells, Cultured DNA / chemistry* DNA / genetics DNA / metabolism Fluorescence Fluorescent Dyes / chemistry* HeLa Cells Humans Mice Microscopy, Confocal / methods Microscopy, Fluorescence, Multiphoton / methods* Molecular Structure NIH 3T3 Cells Organelles / chemistry* Organelles / metabolism Staining and Labeling / methods Time-Lapse Imaging / methods |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| ヒト・動物細胞 | C6(RCB2854) NIH3T3(RCB1862) HeLa(RCB0007) |