RRC ID 63333
著者 Hideaki Shiraishi, Akiko Toyoda
タイトル The use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide–based colorimetric assay in the viability analysis of the filamentous cyanobacterium Arthrospira platensis
ジャーナル Bioscience, Biotechnology, and Biochemistry
Abstract The applicability of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to an industrially valuable filamentous cyanobacterium Arthrospira platensis was examined. When it was applied to A. platensis NIES-39, as few as 10 viable trichomes were quantitatively detected. However, depending on the experimental conditions, it also generated artifactual viability signals. The results should help clarify the scope and limits of the MTT assay in viability analysis.
公開日 2020-12-31
DOI 10.1093/bbb/zbaa050
IF 1.516
リソース情報
藻類 NIES-39