論文 - 詳細
| RRC ID | 63365 |
|---|---|
| 著者 | Matsuda S, Kikkawa U, Nakashima A. |
| タイトル | The S. pombe CDK5 Orthologue Pef1 Cooperates with Three Cyclins, Clg1, Pas1 and Psl1, to Promote Pre-Meiotic DNA Replication. |
| ジャーナル | Biomolecules |
| Abstract |
Meiosis is a specialized cell division process that mediates genetic information transfer to the next generation. Meiotic chromosomal segregation occurs when DNA replication is completed during the pre-meiotic S phase. Here, we show that Schizosaccharomyces pombe Pef1, an orthologue of mammalian cyclin-dependent kinase 5 (CDK5), is required to promote pre-meiotic DNA replication. We examined the efficiency of meiotic initiation using pat1-114 mutants and found that, meiotic nuclear divisions did not occur in the pef1Δ pat1-114 strain. Deletion of pef1 also suppressed the expression of DNA replication factors and the phosphorylation of Cdc2 Tyr-15. The double deletion of clg1 and psl1 arrested meiotic initiation in pat1-114 mutant cells, similar to that of pef1-deficient cells. Meiotic progression was also slightly delayed in the pas1-deficient strain. Our results reveal that Pef1 regulates cyclin-coordinated meiotic progression. |
| 巻・号 | 11(1) |
| 公開日 | 2021-1-12 |
| DOI | 10.3390/biom11010089 |
| PII | biom11010089 |
| PMID | 33445784 |
| PMC | PMC7828282 |
| MeSH | Chromosomes, Fungal / genetics Cyclin-Dependent Kinase 5 / chemistry* Cyclin-Dependent Kinase 5 / metabolism Cyclins / metabolism* DNA Replication* Gene Deletion Meiosis* Models, Biological Phosphorylation Protein Binding Schizosaccharomyces / cytology* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid* |
| IF | 4.082 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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