| RRC ID |
63365
|
| 著者 |
Matsuda S, Kikkawa U, Nakashima A.
|
| タイトル |
The S. pombe CDK5 Orthologue Pef1 Cooperates with Three Cyclins, Clg1, Pas1 and Psl1, to Promote Pre-Meiotic DNA Replication.
|
| ジャーナル |
Biomolecules
|
| Abstract |
Meiosis is a specialized cell division process that mediates genetic information transfer to the next generation. Meiotic chromosomal segregation occurs when DNA replication is completed during the pre-meiotic S phase. Here, we show that Schizosaccharomyces pombe Pef1, an orthologue of mammalian cyclin-dependent kinase 5 (CDK5), is required to promote pre-meiotic DNA replication. We examined the efficiency of meiotic initiation using pat1-114 mutants and found that, meiotic nuclear divisions did not occur in the pef1Δ pat1-114 strain. Deletion of pef1 also suppressed the expression of DNA replication factors and the phosphorylation of Cdc2 Tyr-15. The double deletion of clg1 and psl1 arrested meiotic initiation in pat1-114 mutant cells, similar to that of pef1-deficient cells. Meiotic progression was also slightly delayed in the pas1-deficient strain. Our results reveal that Pef1 regulates cyclin-coordinated meiotic progression.
|
| 巻・号 |
11(1)
|
| 公開日 |
2021-1-12
|
| DOI |
10.3390/biom11010089
|
| PII |
biom11010089
|
| PMID |
33445784
|
| PMC |
PMC7828282
|
| MeSH |
Chromosomes, Fungal / genetics
Cyclin-Dependent Kinase 5 / chemistry*
Cyclin-Dependent Kinase 5 / metabolism
Cyclins / metabolism*
DNA Replication*
Gene Deletion
Meiosis*
Models, Biological
Phosphorylation
Protein Binding
Schizosaccharomyces / cytology*
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / metabolism*
Sequence Homology, Amino Acid*
|
| IF |
4.082
|
| リソース情報 |
| 酵母 |
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