| RRC ID |
63385
|
| Author |
Liu X, Kwong A, Sihoe A, Chu KM.
|
| Title |
Plasma miR-940 may serve as a novel biomarker for gastric cancer.
|
| Journal |
Tumour Biol
|
| Abstract |
It was reported that circulating microRNAs could be applied as non-invasive biomarkers for cancer monitoring. The purpose of this study was to identify plasma miRNA that may serve as a novel biomarker for gastric cancer and to evaluate its clinical application. MicroRNA profiles were generated from plasma samples of 5 patients with gastric cancer (GC) versus 5 healthy controls (HC). MicroRNA-940 (miR-940) was one of the most downregulated miRNAs with fold change of 0.164. It was revealed that the expression of miR-940 was downregulated in both the initial set (N = 30, P < 0.0001) and the validation set (N = 80, P < 0.0001) of plasma samples of patients with gastric cancer. The sensitivity was obviously higher than the current biomarkers CEA and CA19-9 (81.25 % vs. 22.54 % and 15.71 %). MiR-940 was also significantly downregulated in gastric cancer tissue samples (N = 34, P = 0.0015), as well as in cancer cell lines (N = 7). Importantly, miR-940 was significantly highly expressed in stomach tissue samples than in other types of tissue samples including the liver, breast, thyroid, and lung. Moreover, there was a trend of lower expression of miR-940 from early to advanced stage of gastric cancer. Target prediction suggested that miR-940 regulated cell signaling including NF-κB and Wnt/β-catenin, as well as pathways of cell communication and adhesion. These pathways play critical roles in gastric cancer initiation and progression. It is the first report that miR-940 may mainly express in the stomach. Downregulation of plasma miR-940 may serve as a novel biomarker for detection of gastric cancer.
|
| Volume |
37(3)
|
| Pages |
3589-97
|
| Published |
2016-3-1
|
| DOI |
10.1007/s13277-015-4019-5
|
| PII |
10.1007/s13277-015-4019-5
|
| PMID |
26456959
|
| MeSH |
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor / blood
Biomarkers, Tumor / genetics*
Cell Line, Tumor
Down-Regulation
Female
Gene Expression Profiling / methods*
Gene Expression Regulation, Neoplastic*
Humans
Male
MicroRNAs / blood
MicroRNAs / genetics*
Middle Aged
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / blood
Stomach Neoplasms / genetics*
Stomach Neoplasms / pathology
|
| IF |
3.65
|
| Resource |
| Human and Animal Cells |
MKN7(RCB0999)
MKN28(RCB1000)
MKN45(RCB1001) |
