論文 - 詳細
| RRC ID | 63440 |
|---|---|
| 著者 | Ozeki N, Mogi M, Hase N, Hiyama T, Yamaguchi H, Kawai R, Kondo A, Nakata K. |
| タイトル | Wnt16 Signaling Is Required for IL-1β-Induced Matrix Metalloproteinase-13-Regulated Proliferation of Human Stem Cell-Derived Osteoblastic Cells. |
| ジャーナル | Int J Mol Sci |
| Abstract |
We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7⁺hSMSC)-derived osteoblast-like (α7⁺hSMSC-OB) cells, and found that interleukin (IL)-1β induces matrix metalloproteinase (MMP)-13-regulated proliferation of these cells. These data suggest that MMP-13 plays a potentially unique physiological role in the regeneration of osteoblast-like cells. Here, we examined whether up-regulation of MMP-13 activity by IL-1β was mediated by Wingless/int1 (Wnt) signaling and increased the proliferation of osteoblast-like cells. IL-1β increased the mRNA and protein levels of Wnt16 and the Wnt receptor Lrp5/Fzd2. Exogenous Wnt16 was found to increase MMP-13 mRNA, protein and activity, and interestingly, the proliferation rate of these cells. Treatment with small interfering RNAs against Wnt16 and Lrp5 suppressed the IL-1β-induced increase in cell proliferation. We revealed that a unique signaling cascade IL-1β→Wnt16→Lrp5→MMP-13, was intimately involved in the proliferation of osteoblast-like cells, and suggest that IL-1β-induced MMP-13 expression and changes in cell proliferation are regulated by Wnt16. |
| 巻・号 | 17(2) |
| ページ | 221 |
| 公開日 | 2016-2-6 |
| DOI | 10.3390/ijms17020221 |
| PII | ijms17020221 |
| PMID | 26861315 |
| PMC | PMC4783953 |
| MeSH | Antigens, CD / genetics Antigens, CD / metabolism Cell Differentiation / genetics Cell Proliferation / drug effects Frizzled Receptors / genetics Frizzled Receptors / metabolism Gene Expression Regulation / drug effects Gene Silencing Humans Integrin alpha Chains / genetics Integrin alpha Chains / metabolism Interleukin-1beta / drug effects Interleukin-1beta / metabolism* Low Density Lipoprotein Receptor-Related Protein-5 / genetics Low Density Lipoprotein Receptor-Related Protein-5 / metabolism Matrix Metalloproteinase 13 / genetics Matrix Metalloproteinase 13 / metabolism* Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism* RNA Interference RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / genetics Signal Transduction* / drug effects Stem Cells / cytology Wnt Proteins / genetics Wnt Proteins / metabolism* |
| IF | 4.556 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |