論文 - 詳細
| RRC ID | 63453 |
|---|---|
| 著者 | Futawaka K, Tagami T, Fukuda Y, Koyama R, Nushida A, Nezu S, Imamoto M, Kasahara M, Moriyama K. |
| タイトル | Growth hormone regulates the expression of UCP2 in myocytes. |
| ジャーナル | Growth Horm IGF Res |
| Abstract |
OBJECTIVE:To determine if and how growth hormone (GH) signaling is involved in energy metabolism. DESIGN:We used human embryonic kidney TSA201 cells, human H-EMC-SS chondrosarcoma cells, rat L6 skeletal muscle cells, and murine C2C12 skeletal muscle myoblasts to investigate GH-induced expression of uncoupling protein2 (UCP2) to the GHR/JAK/STAT5 pathway by a combination of a reporter assay, electrophoretic mobility shift assay (EMSA), real-time quantitative PCR, Western blotting. RESULTS:We demonstrated that the regulation energy metabolism, which was hypothesized to be directly acted on by GH, involves UCP2 via activated STAT5B, a signal transducer downstream of GH. We also showed that the sequence at the -586 'TTCnGA' may function as a novel putative consensus sequence of STAT5s. CONCLUSION:The results suggest that GH regulates energy metabolism directly in myocytes and that UCP2 participates in the signal transduction pathway that functions downstream of the GHR/JAK/STAT. |
| 巻・号 | 29 |
| ページ | 57-62 |
| 公開日 | 2016-8-1 |
| DOI | 10.1016/j.ghir.2016.04.005 |
| PII | S1096-6374(16)30034-X |
| PMID | 27150070 |
| MeSH | Animals Blotting, Western Cell Line, Tumor Chondrosarcoma / genetics Chondrosarcoma / metabolism* Electrophoretic Mobility Shift Assay Energy Metabolism / genetics* Gene Expression Regulation Gene Expression Regulation, Neoplastic Growth Hormone / metabolism* HEK293 Cells Humans Janus Kinase 2 / metabolism* Janus Kinases / metabolism Muscle Fibers, Skeletal / metabolism* Rats Real-Time Polymerase Chain Reaction Receptors, Somatotropin / metabolism STAT5 Transcription Factor / metabolism* Signal Transduction Uncoupling Protein 2 / genetics* |
| IF | 1.618 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |